Analysis The Antibiotic-resistance And Resistance Gene Of Esxherichia Coli In The Elder's Hospital Of Shaoxing

Escherichia coli was one of the most common pathogen in our hospital, in order to understande the antibiotic-resistence change of Escherichia coli and provide new information for doctors, we isolated 58 strains Escherichia coli in 2004 and 74 strains of Escherichia coli in 2005. The antibiotic-resistence results showed that in 2004, 82.5% of Escherichia coli were resistence to Tetracycline, 72.4% of Escherichia coli were resistence to broad-spectrum Penicillin such as Amoxicillin and Piperacillin and more than 50% of Escherichia coli was resistence to Amikacin, Gentamycin, Tobramycin, Ciprofloxacin. 70.7%, 43.1% and 31.0% of Escherichia coli were resistence to third generation of Cephalosporins such as Cefotaxime, Ceftriaxone and Cefatazidime respectively. Only 6.9% Escherichia coli were resistence to Cefoperazone/Sulbactam and 5.2% of Escherichia coli were resistence to Piperacillin/Tazobactam. 100% of Escherichia coli were sensitive to Imipenem. In 2005, 82.4% of Escherichia coli were resistence toTetracycline, and more than 80% of Escherichia coli were resistence to Amoxicillin and Piperacillin. 74.3% of Escherichia coli were resistence to Ciprofloxacin. More than 50% of Escherichia coli were resistence to Amikacin, Gentamycin, Tobramycin. 79.9%, 47.3% and 35.1% of Escherichia coli were resistence to Cefotaxime, Ceftriaxone and Cefatazidime respectively. And 10.8%, 6.8% and 1.4% of Escherichia coli were resistence to Cefoperazone/Sulbactam, Piperacillin/Tazobactam and Imipenem respectively.In 2004, 34.5% (20/58) Escherichia coli were Extended-Spectrum B-Lactamases (ESBLs) positive in our hospital. In 2005, 41.3% (35/74) of Escherichia coli were ESBLs positive, which was siginificantly higher than that of in 2004 in our hospital. Furthermor PCR results showed that 26 strains of Escherichia coli were CTX-M14 positive in all ESBLs positive strains, 10 strains were TEM positive, 4 were CTX-M1 positive, 3 were SHV positive.Meanwhile, in order to investigate the characteristic of lung infection in elder patients and provide new useful information for doctors, we analyses bacterial and antibiotic resistance change of elder lung infection patients in Shaoxing elder hospital from Oct. 1999 to Oct. 2004. 400 cases of sputum from elder lung infection patients were collected, we isolated 96 strains of bacterial, the total positive rate was 24.0%. 73.9% of thesebacterial were Gram negative strains. The most common bacteria was Pseudomonas aeruginosa, which account for 19.8% of total bacterial, the second and third most common bacterial were Klebsiella pneumoniae and Enterobacter cloacae. 21.9% were Gram positive bacterial, among these the main bacterial were Coagulase Negative Staphylococcus(CNS), Enterococcus and Staphylococcus aureus. 4 strains of Candidia albicans were also isolated, which account for 4.2% of total bacterial. Meanwhile we analyses antibiotic resistance rate of these bacterial. Pseudomonas aeruginosa to Cefazolin, Cefotaxime and Ceftazidime resistence rate was 84.2%, 57.9% and 5.3% respectively. Klebsiella pneumoniae to Cefazolin, Cefotaxime, Ciprofloxacin and Amikacin resistence rate was 57.1%, 57.1%, 35.7% and 50% respectively. Enterobacter cloacae to Cefazolin, Ciprofloxacin and Imipenem resistence rate was 78.4%, 41.7% and 18.3% respectively. Coagulase Negative Staphylococcus(CNS) to Oxacillin, Ciprofloxacin, Penicillin G resistence rate was 71.4%, 57.1% and 85.7% respectively.