| Adhesion molecules are this kind of molecules which are generated by cells and can mediate the touch and conjugate between cell and cell or cell and cell matrix, including mainly: ①immunoglobulin superfamily;②integrin family;③selectin superfamily;④cadherins family;⑤others not elsewhere classified. intercelluar adhesion molecular-1(ICAM-1), and Vascular cell adhesion molcular-1(VCAM-1) both belong to the immunoglobulin superfamily. ICAM-1 is diffusely expressed in the leucocytes, vascular endothelial cells, mononuclear macrophage, and lymphocytes;and VCAM-1 is mainly expressed in the activated endothelial cells, monocytes, epithelial cells. And both can be induced to express by many kinds of factors. Those factors include that IL-1,TNF-α ,PAF,IFN- γ ,hyperlipemia, diabetes, and hypertension etc. ICAM-1and VCAM-1 together with selectin and integrin, mediate leucocytes' adherence, rolling, and attachment, and then transmigrating out of the blood vessel which at last leads to inflammation reaction. sICAM-1 and sVCAM-1 is extracelluar portion of the membrane-bound form, the products of enzymolysis or the direct products of differential splicing, and both have the activity of adhesion molecules. Through detecting the concentration of sICAM-1 and sVCAM-1 in the serum, we studied the different concentration of sICAM-1 and sVCAM-1 in different point in time in the patients with cerebral hemorrhage.In this study, test kits purchased from the company of DIACLONE (FRANCE), with antibody coating on the solid phase carrier (96-well Microplate), and then all the experimental operations were strictly under the direction of the guideline. At last, with the help of the software of BIORAD, we can get the data.The results of the study are shown below: (Dthe serum levels of sICAM-1 and sVCAM-1 have significant differences between the group the acute cerebral hemorrhage and the control at the different points in time.(2)the serum levels of sICAM-1 and sVCAM-1 of the group the acute cerebral hemorrhage compared with the control at the different points in time: rose higher than those of control but without significant differences in the first day;increased gradually to the level higher than those of the control with significant differences in the 3nd and 7th day;inthe 14th day began to decrease, but still remained higher than the control with significant differences.(3)after removing the effect of hypertension history, the levels of sICAM-1 in the 3rd day and the levels of sICAM-1 and sVCAM-1 in the 7th day were significantly different between the mild cases and the severe cases.(Dthe serum levels of the sICAM-1 and sVCAM-1 have statistically differences among the mild cases and the severe cases in the 3rd day and the 7th day;and the serum levels of the sVCAM-1 in the 3rd day and of the sICAM-1 and sVCAM-1 in the 3rd day and the 7th day have statistically differences between the group with the small haematoma and the large haematoma.Through the study, we find that: the serum levels of the sICAM-1 and sVCAM-1 of the cerebral hemorrhage began to rise in the first day, reached the peak in the 7th day, and remained in the high level till 14th day, which suggested that there was inflammatory reaction in the brain tissue around haematoma in the patients with cerebral haemorrahge. Furthermore, the serum levels of the sICAM-1 and sVCAM-1 have statistically differences among the mild cases and the severe cases in the 3rd day and the 7th day, and the serum levels of the sICAM-1 and sVCAM-1 in the 3rd day and the 7th day have statistically differences between the group with the small haematoma and the large haematoma. These suggested that the raised serum levels of sICAM-1 and sVCAM-1 might be related to the severity of the disease and the haemorrahge size.We believed that after cerebral haemorrahge, inflammatory reaction might play a certain role in the secondary injury of the brain tissue. |
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