Effects Of Gisenoside-Rg1 On BDNF Protein In The Protection Against Brain Ischemia

Objective: To explore the effects of gisenoside-Rgi on BDNF (brain-derivedneurotrophic factor) protein in the protection against brain ischemia, as well as toinvestigate whether gisenoside-Rgi can adjust the content of BDNF, precursor tactics with the positive neurons figure or downstream tactics etc. Methods: SD rats, weighing 280-320 g, were randomly divided into groups and their frozen brain sections (slicethickness 12 urn). The sections were stained using specific BDNF (1:500) antibody underthe same condition according to the immunohistochemistry ABC method. The BDNF content and positive neurons in cerebrum cortex, hippocampus, cerebelli and Medulla oblongata in each animal were observed and counted. Data were analyzed one-wayAnova and q test. Results : The results showed that : (1) Gisenoside-Rgi could obviouslyimprove some nervous deficit symptoms in the brain ischemia. The curative effect was superior to that of the reference drug (nimodipine), and better at the dose of 100 mg/kg and50 mg/kg. Gisenoside-Rgi was marketable in the 3-7 d of treatment period too. (2)Gisenoside-Rgi could increase BDNF's protein content and BDNF positive neurons amount in the cerebrum cortex in rat brain ischemia. Gisenoside-Rgi was powerful in the former, but had no difference in the treatment period. The efficacy of Gisenoside-Rgi was similarto that of nimodipine, and better on d 7 than others. Gisenoside-Rgl, at the doses of 50 mg/kg and 100 mg/kg, showed more potent effects than that of 200 mg/kg (3 ) There wassignificance in the hippocampus at the difference time between the above three doses. The efficacy of gisenoside-Rgi was higher at 50 and 100 mg/kg than 200 mg/kg, better in the treated period of d 3 and d 7. In total, gisenoside-Rgi could increase BDNF's protein content and BDNF positive neurons amount in the hippocampus. The curative effect was superior or similar to that of the reference drug (nimodipine) in the different groups. (4)There showed differences between the doses at the different time in the Cerebelli, showing more potent effects than nimodipine at 50 and 100 mg/kg. Gisenoside-Rgi wasmarketable in the treatment period of 3-7 d. Total tendency displayed: gisenoside-Rgicould increase BDNF's protein content and BDNF positive neurons amount in cerebrum cortex. The curative effect was superior to that of the reference drug (nimodipine). (5) Gisenoside-Rgi increased medulla oblongata BDNF's protein content and BDNF positive neurons amount, and showed a higher effect at 100 mg/kg and 50 mg/kg. Gisenoside-Rgi at 100 mg/kg and 50 mg/kg, was marketable in the treatment period of 3-7 d. Thecurative effect was superior to that of nimodipine. Conclusion: Gisenoside-Rgi couldupper-adjust BDNF protein content and positive neurons of the BDNF amount in the cerebrum cortex, hippocampus, cerebelli, and medulla oblongata.