Effect Of Ramipril On Expression Of NF-(?)B In Acute Lung Injury

Objective To observe changes of the expression of NF- κB in the lung tissue and IL-1β in plasma in lipopolysaccharide(LPS)-induced acute lung injury(ALI), and influence of Ramipril on NF-κB , IL-1β and ALLMethods 84 SD rats were divided randomly into three groups:control group,LPS group and RAM group(n=28each). LPS group received LPS(2mg/ml,6mg/kg)by intra-peritoneal injection to make the model of ALI. RAM group received Ramipril (70ug/kg)and LPS(6mg/kg)l hour later. Control group were given 0.9% NaCl solution(3ml/kg). After 0,1,3 and 5 hours, the plasma concentration of IL-1β in rats was measured by radioimmunoassay. After 3 hours, the expression of NF-κB in the lung tissue was measured by immune histochemistry, and took count of inflammatory cell in the lung tissue. At last, the pathomorphologic change of lung tissue were examined.Results ( 1 ) Compared with the control group, the plasma concentration of IL-1β in the LPS group at the end of 1 hour elevated significantly (P<0. 05), The plasma concentration of IL-1β at the end of 1 hour was significantly lower in the RAM group than in the LPS group (P<0.05). (2) The expression of NF-κB in the LPS group was significantly higher than that in the control group, (P<0.05), the inflammatory cell in the lung was higher than in the control group(P<0.05), and the pathomorphology in lung tissue showed sever injuries. Compared with the LPS group, the expression of NF-κB in the RAM group was significantly lower(P<0.05), the inflammatory cell in the lung was lower than in the LPS group(P<0. 05), and the lung injury was less, too.Conclusions NF-κB plays a critical role in the LPS-induced ALI .Ramipril has an antagoniging effect on IL-lP,may lower the expression of NF-k B and relieve LPS-induced ALL...