The Study Of Ang-2 And VEGF In The Angiogenesis Of Colorectal Carcinoma

Background and Objective: Colorectal carcinoma is the one of the most comment malignant neoplasm in our country. The important dead reason of colorectal carcinoma is its metastasis. It is possible to control the colorectal carcinoma metastasis and growth by inhibiting the angiogenic growth factor tumor.The regulation of angiogenesis of tumor was countroled by the angiogenesis growth factor and angiogenesis inhibition factors. There are more factors that can affect the angiognasis of tumor. The recently study indicated that the ang and VEGFare more important factor of angiogenesis of tumor. Angiopoietin-2 is a new-cloned molecular of angiogenic growth factor, which is tightly relation to tumor angiogenesis. VEGF is a factor of vascular endothelial growth; it can improve the tropism of vascular endothelial cell and stimulate the division of vascular endotheial cell and the pullulation and growth of micrangium. The both of Ang-2 and VEGF co-operate in the tumor angiogenesis and improve the tumor angiogenesis. The aim of this study is to explore the mutual relationship of Ang-2 and VEGF and their influence on invasion and metastasis of colorectal carcinoma by detecting the expression of Ang-2 and VEGF on colorectal carcinoma.Methods: All the samples were collected from the first Affiliated Hospital of Zhen Zhou University in 2003.Ang-2 and VEGF were detected by immunohistochemical in 40 cases of colorectal carcinoma and normal mucosa and use CD34 as a marker to calculate the MVD. We observe their expression to explore their mutual relation to analysis their influence metastasis of colorectal carcinoma and the relation between the expressions of Ang-2 and VEGF and the clinic pathologic parameters of colorectal carcinoma. All the date was analyzed by SPSS 10.0 statistical package. Thecomparison of positive rates was analyzed by Chi-Square t test and the relation of two variable were analyzed by the correlation analysis. The level of significant difference is a =0.05.Result: 1 the expression of Ang-2 was 25 cases in 40 cases of colorectal carcinoma, the positive rare was62.5 %( 25/40); There were 5 cases of Ang-2 was expressed on the normal colorectal mucosa. The positive rate was 12.5 %( 5/40). The both of positive rates of Ang-2 on the colorectal carcinoma was higher than their on the normal colorectal mucosa, there was significant difference (P <0.05) . (2) There was20 cases was expressed in the 24 cases of colorectal carcinoma with lympth node metastasis, but 5 cases in the 16 cases of colorectal carcinoma without lympth node metastasis. There was significant difference (P <0.05) ,the positive rates of Ang-2 was 84.0%(21/25) in the group of colorectal carcinoma with serosa invaded and 26.11%(4/15) in the group of colorectal carcinoma without serosa invaded, there was significant difference (P <0.05) . (3)the positive rata of Ang-2 was 48.0%(12/15) in the group of middle-well differentiation colorectal carcinoma and 80.67%(13/15) in the poor differentiation colorectal carcinoma, there was significant difference(P < 0.05).(4) The expression of Ang-2 was not correlation to the the size, clinical Duck' s classification of tumor and the ages, sexs of the patients.2 The expression of VEGF was28cases in 40 cases of colorectal carcinoma, the positive rare was62.5 %( 25/40); There was 1 cases of VEGF was expressed in 40 cases of the normal colorectal mucosa, the positive rates was0.25 %( 1/40). The positive rates of VEGF on the colorectal carcinoma was higher than their on the normal colorectal mucosa, there was significant difference (P <0.05) . (2) There was21 cases was expressed in the 24 cases of colorectal carcinoma with lympth node metastasis, but 4 cases in the 16 cases of colorectal carcinoma without lympth node metastasis. There was significant difference (P <0.05) . (3) The expression of VEGF was 11 cases in 25 cases of the middle-well differentiation cancers and 14 cases in the 15 cases of poor differentiation cancers. There was significant difference (P <0.05) , the positive rates of VEGF was 76.0%(19/25) in the group of colorectal carcinoma with serosa invaded and 40.0%(6/15) in the group of colorectal carcinomawithout serosa invaded, there was significant differenec (P <0.05),The expression of VEGF was not correlation to the size, clinical Duck' s classification of tumor and the ages, sex of the patients.3 The MVD was 34.4+13.90 in the 40 cases with colorectal carcinoma andl8.96+ 13.90 in the 40 cases with normal colorectal mucosa, there was significant difference (P <0.05 ) .The MVD was41.43 +11.38 in the group of colorectal carcinoma with the positive expression of Ang-2 and 22.68 + 9.003 in the group of colorectal carcinoma with the negative expression of Ang-2, there was significant difference (P <0.05) ; The MVD was 41.33 + 11.61 in the group of colorectal carcinoma with the positive expression of VEGF and 22.84+8.88 in the group of colorectal carcinoma with the negative expression of VEGF, there was significant difference (P <0.05 ) ;4 There were 23 cases of Ang-2 expressed in the 25 cases of positive expression of VEGF and 2 cases of Ang-2 expressed in the 15 cases of negative expression of VEGF. There was significant correlation between the expression of Ang-2 and VEGE. rs=0.787,P <0.05.Conclusions:1 The expression of Ang-2 was higher than those in the normal colorectal mucosa. The MVD of colorectal of carcinoma tissue with Ang-2 positive expression was higher than it in the colorectal of carcinoma tissue with the Ang-2 negative expression. It is possible that Ang-2 can stimulate the angiogenesis of colorectal carcinoma, and then improve the development of colorectal carcinoma.2 The expression of VEGF was higher than those in the normal colorectal mucosa. The MVD of colorectal of carcinoma tissue with VEGF positive expression was higher than it in the colorectal of carcinoma tissue with the VEGF negative expression. VEGF was tightly related to the angiogenesis of colorectal carcinoma.3 The expression of Ang-2 was significant correlation to the expression of VEGF; both of them contribute y to the angiogenesis of colorectal carcinoma and improve possibly the development of colorectal carcinoma.4 Ang-2 and VEGF were tightly related to the metastases of colorectalcarcinoma.