Anti-tumor And Immunoregulatory Activities Of Polysaccharides From The Sea Cucumber On The Tumor-bearing Mice

We　generated　the　mouse　hepatic　tumor　model　and　randomize　them　into　groups.　Control　or　chemicals　are　administrated　by　injection　intraperitonealy　or　by　gavage　and　the　rate　of　anti-tumor　effect　and　spleen　index　were　calculated.　We　also　determined　the　effect　of　polysaccharides　from　the　sea　cucumber　on　the　macrophage　function　of　spleen　macrophages　and　measured　the　tumor　kill　activity　of　spleen　macrophages　and　lymphocytes.　And　determined　the　concentration　of　TNF-α　in　the　mice　spleen　macrophages　supernatant　and　the　IL-2　of　the　spleen　lymphocytes.　And　their　anti-tumor　activity　was　observed　by　MTT　assay　using　K562　cells　in　vitro.　Our　results　showed　that　intraperitoneal　administration　of　polysaccharides　from　the　sea　cucumber　exhibited　significant　anti-tumor　effect　in　inhibiting　the　growth　of　mice　tumors　and　the　difference　of　the　spleen　index　is　significant　with　t he　dose　dependent　effect.　There　is　no　significantd　ifference　of　the　tumor　kill　activity　of　spleen　macrophages　and　lymphocytes　and　the　macrophage　function　of　spleen　macrophages　within　groups.　The　TNF　secreting　function　of　the　40mg/kg　group　mice　spleen　marcrophages　have　significant　difference　but　not　of　the　IL-2　secreting　function　of　the　spleen　lymphocytes.　The　effect　of　polysaccharides　from　the　sea　cucumber　by　gavage　on　the　anti-tumor　effect　in　inhibiting　the　growth　of　mice　tumors　and　the　spleen　index　is　not　significant　in　all　the　groups.　There　are　significant　difference　of　the　tumor　kill　activity　of　the　200,400mg/kg　group　mice　spleen　macrophages,　but　no　of　the　tumor　kill　activity　of　the　spleen　lymphocytes.　The　100,200,400mg/kg　groups　macrophage　function　of　spleen　macrophages　have　significant　difference.　The　combined　treatment　group　has　no　significant　difference.　The　　macrophages　function　of　spleen　macrophages　within　normal　medication　group,　tumor　model　group　and　normal　control　group.　The　polysaccharides　from　the　sea　cucumber　exhibited　significant　anti-tumor　(K562)　effect　in　inhibiting　the　growth　of　mice　tumors　in　vitro.　In　summary,　the　polysaccharides　from　the　sea　cucumber　showed　significant　inhibiting　effect　on　mice　hepatic　tumor.　The　mechanism　of　the　tumor　inhibiting　effect　is　related　with　the　enhancement　of　the　immuno　activity.