Effect Of Mu Dan Granule On Islet Cell Apoptosis In Diabetic Rats And Its Mechanism

Purpose:This experiment is designed to study the protective effect of islet cells diabetic rats of Mudangranule with streptozotocin (STZ)-induced diabetes. Observation islet β cellsTNF-a,Caspase-3,NF-κB protein expression, and explore the possible mechanism of Mudangranule islet cell reducing apoptosis.Methods:80male SD rats were randomly select10for the normal group, and the remaining70instreptozotocin (STZ)53mg/kg dose of a single injection modeling.，The rats whose bloodglucose is no less than16.7mmol/L in the model were chosen rats and divided into modelgroup, Mudan granula low-dose group（1.15g/kg/d）,middle dose group Mudan granula（2.3g/kg/d）, high dose of Mudan granula group（4.6g/kg/d）and glargine group（4U/kg/d）at random, the number of rats in each group was8. The dose groups were given wood particleaccording to the corresponding concentration, dosing volume was10ml/kg, once a day, forfour consecutive weeks, the normal group and model group were given the same volume ofdistilled water. Glargine group were injected subcutaneously4U/kg daily for4weeks. TheFBG of rats were determined after administered2weeks and4weeks, when the weight ofrats were measured, taking pancreatic tissue of rats after anesthesia, and HE staining ofpancreatic to observation tissue morphology. The expression of cell death signals TNF-α,NF-κB, Caspase-3in the islet β with immunohistochemical detection.Results:①Model rats with normal fasting glucose group was significantly higher (p <0.01);comparedwith the model group, Mudan treatment group no significant decrease in FBG (P>0.05),glargine group decreased significantly (p<0.01);②HE staining of pancreatic tissue pathology,compared with the model group, diabetic rat pancreatic islet cell apoptosis Mudan granule ofSTZ-induced particle group has a better effect;③immunohistochemistry results showed that, the expression of TNF-α, NF-κB, Caspase-3can be dropped in Mudan granule and glargine.Conclusion:Mudan granule can reduce the expression of TNF-α，NF-κB，Caspase-3in the islet cells, andwith less apoptosis, which can protect the pancreatic islet cells.