Research Of PDGF Antibody VEGF Antibody AG1295 Inhibiting Proliferative Vitreoretinopathy

Proliferative vitreoretinopathy(PVR) is a disease process that occurs ineyes with rhegmatogenous retinal detachments and the complication of retinaldetachment surgery. Owing to trauma ,there will be proliferation of fibroustissue--traumatic PVR.PVR accounts for the majority of failures followingretinal detachment surgery. Retinal pigment epithelial (RPE) cells play apivotal role in the pathogenesis of proliferative vitreoretinopathy (PVR).TheStimulaition of inflammatory factors,RPE cells free,migrate,proliferate fromthe slit pore of retina, form proliferatove membrane in vitreous,superretinaand subretina,contracte, result in PVR.There are some factors in thepathogenesis of PVR,ep: platelet derived growth factor(PDGF),vascularrendothelial growth factor(VEGF),hepatocyte growth factor(HGF),matrixmetallo proteinases(MMP),tumor necrosis factor(TNF),pigment epitheliumderived factor(PEDF),fibroblast growth factor(FGF),transforming growthfactor(TGF),interleukin-1 interleukin interleukin and so on.The inhibition ofPVR is still the popular subject of ophthalmology.Now there are theremethods:drug,operation,gene therapy. Cutting proliferative tissue is maijormethod to inhibit PVR.Although vitrectomy has developed perfectly now,theeffectis not satisfactory.In this case,drugs inhibition of PVR has beenemphasized.At present, vitrectomyis still main method to inhibiting PVR,butthere are some recurrences of proliferative menmbrane in cases which haveserious proliferative vitreoretinopathy.Retinal detachment recur,patient isoperated secondary. Because of the radical operation,stimulation of siliconeoil ,postoperative inflammation, proliferative menmbrane recur. In theprevention of PVR,drugs are applicated when perfusing in PPV. The article plays special emphases upon PDGF and VEGF.PDGF isimportant in the chemotaxis and proliferation of RPE and glial cells, result inepiretinal membranes and subretinal membranes.The PDGF concentration invitreous of the patient associated with PVR is higher than that not associatedwith PVR,therefore it is applicable to injecting PDGF antibody tovitreous.VEGF is a multifunctional factor which accelerate neovascularizationand migrate,cleavage,proliferate RPE cells. Ozaki has maken advancement tocure neovascularization with inhibiting VEGF and its receptor.Some data showthat cell density of on vascular retinal proliferative membranes caused by PVRas lower than cell density in vascular retinal proliferative membranes causedby PDR .This implies that the treatment of PVR can not simply depent onthe inhibition of cell proliferation.So the prevent to neovascularizationis alsoimportant. The adult pigmented rabbits are experimentalanimal that they are injuredto form PVR. Adult pigmented rabbits were given 0.1 mL intravitrealinjections of 200μg/ml PDGF antibody ,200μg/ml VEGF antibody ,Platelet-derived growth factor receptor kinase inhibitor AG1295 at the firstday and the seventh day. Noninjected eyes served as controls.To observe fudusof eyes everyday,taking photos of fudus.On eh basis of Fastenbergstandard,PVR grades are classified. In eyes that received 200μg/ml PDGFantibody,200μg/ml VEGF antibody,Platelet-derived growth factor receptorkinase inhibitor AG1295, the photos of fudus show that sevsrity of PVR arelighter than in controls at the twenty-first and the twenty-eighth day...