The Insular Cortex And Obstructive Sleep Apnea Syndrome Animal Model

It is universally considered that the soft tissue surrounding pharynx repetatcollapes during sleep is the main etiological factor on OSAS.Genioglossus muscleplays an important role in dilate pharyngeal cavity. Innervation and control of5-HT neurons in raphe nucleus to the pharyngeal muscle had been studied toreveal that it ties tightly up with the mechanism of OSAS。METHOD:The experiments were done on urethaneana esthetized, spontaneouslybreathing healthy rats. Animals were randomize into four groups: 20 toL-glutamate(L-Glu) microinjection into the insular cortex. control groups include;5 to artificial cerebrospinal fluid, 5 to microinjection surrounding insular cortexand 5 to pseustimulation. After L-glutamate(L-Glu) microinjection into the insularcortex (INS) of rats, the amplitude and frequency of respiratory movement wereobserved.RESULT:Of all the group which was injected L-Glu into INS, 3/4 rats bursted withapnea lasting several seconds, aferwards the frequency of respiratory movementreduced and amplitude increased. a few seconds or minutes later, thediversification of respiration moved in cycle.The results of microinjection ofL-glutamate into INS were similar to stimulation into raphe nucleus.But comparedwith the curve of premicroinjection, these three control groups hardly altered.CONCLUSION:The above results suggest as follows: on the one hand, we prove that thereare L-Glu neurons in insular cortex; on the other hand, habenula and raphenucleus are the pathways involved in the respiratory movement induced by the...