Expression Of Cancer-Testis Antigen SCP-1 MRNA In Human Nasopharyngeal Carcinoma And Its Significance

Objective: To investigate the expression of Cancer-Testis Antigen SCP-1 mRNA in human nasopharyngeal carcinoma, and explore the potential possibility in the immunodiagnosis and immunotherapy of nasopharyngeal carcinoma with SCP-1.Methods: With reverse transcriptase polymerase chain reaction, the SCP-1 mRNA was examined in the tumor tissues of 28 nasopharyngeal carcinoma cases and control pharynx nasalis tissues of 40 non-cancer cases. A randomized sample was respectively extracted for DNA sequencing from positive electrophoresis expression samples of SCP-1 mRNA to examine the reliability of results. The correlation between the expression of SCP-1 mRNA and clinical data was analyzed by statistical method.Result: The expressive frequency of SCP-1 mRNA was 14.3 %( 4/28) in nasopharyngeal carcinoma. There was no SCP-1 mRNA detected in control pharynx nasalis tissue of non-cancer cases (P<0.05). The DNA sequence confirmed that the reverse transcriptase polymerase chain reaction product was truly the target gene. No significant relationship was found between the expression of SCP-1 mRNA and clinical data, such as age, gender and serum anti-EBV level (P>0.05).Conclusion: SCP-1 mRNA was expressed in nasopharyngeal carcinoma, suggesting that SCP-1 might be a target antigen specific for immunodiagnosis and immunotherapy of nasopharyngeal carcinoma cases.