The Effect Of Oral Malodor System On Identifying Halitosis-correlated Bacteria

To establish a salivary sediment system, which can simulate the metabolism of halitosis formation, and to evaluate the effect of several kinds of bacteria dedicated to the halitosis formation , hence to identify halitosis-correlated bacteria.The irritant whole saliva was obtained from 10 periodontally healthy individuals. First, the proportion of the supernatant and sediment, gained from centrifugal whole saliva, was adjusted to establish an oral malodor system incubated under 37 degrees for 7 hours. Head space and liquid-phase odors were determined organolepticallly, and VSCs and pH were determined by Halimeter and glass electrode respectively between hours to evaluate the malodor formation. Second, the suspended fluid of suspicious halitosis-correlated bacteria and non-halitosis-correlated bacteria and water control were introduced into the oral malodor system incubated for 1 hour. The five groups were incubated under 37 degree in the residual hours. Odors, VSCs and pH changes were recorded between hours to evaluate the halitosis formation caused by the bacteria.The oral malodor system can simulate the metabolism of halitosis formation to produce odor after the proportion of the supernatant and sediment of the saliva was adjusted to establish it. The odors of the suspicious halitosis-correlated bacteria introduced groups were higher than the non-halitosis-correlated bacteria and water control group, especially the P.gingivalis group. With its simple but fundamental manipulations, incubated whole saliva is a powerful model for study of the metabolism of the oral mixed microbial flora, malodor formation, and other oral diseases-related processes. To remodel the microbial load by change the number of halitosis-correlated bacteria will have major effects on bacterial metabolic activity and hence on malodor. Thus, we can use this model to identify the halitosis-correlated bacteria.