Studies On Analysis Of Volatile Sulphur Compounds In Mouth Air

Halitosis is a condition in which objectionable odors are present in mouth air. Most patients perceived this condition as primarily a cosmetic or social intercourse problem. Between 50 and 60 per cent of the population suffered from halitosis. This can have consequences for private or professional life, evenly induced psychoses. And an increasing volume of evidence was demonstrating that halitosis were highly associated with the initiation and development of oral diseases, such as gingivitis, perodontitis. Volatile sulfur compounds (VSCs) were a family of gases which were primarily responsible for halitosis. Extremely low concentrations of these compounds, such as hydrogen sulfide (H2S) and methyl mercaptan (CH3SH), were highly toxic to tissues. Therefor, VSCs can initiate or aggravate oral diseases.In this study, we used a gas chromatography equipped with a pulse flame photometric detector to estimate the main ingredients of volatile sulfur compounds in mouth air of patients of halitosis, and assessed the relationship between VSCs levels and periodontitis. The correlation between two measurements of VSCs in mouth air: halimeter and gas chromatography was demonstrated. To probing into adjunct therapeutic method to halitosis efficiently, we examined the oral malodor-inhibiting effect of zinc sulphate solution.Part I: A Study on Analysis of Volatile Sulphur Compoundsin Mouth Air in Patients with Halitosis by GasChromatography[Materials and Methods ]Fifty patients of halitosis (mean age 37 years; 23 males, 27 females) were randomly selected in halitosis clinic in our hospital. They were instructed to abstain from oral hygiene, including oral rinsing, and ingestion of food and liquid on the morning of the test. Experiments were performed between 8:00 and 9:00 a.m. The VSCs analysis included a CP-3800 gas chromatography equipped with a pulse flame photometric detector. We used it to estimate the main ingredients of volatile sulfur compounds in mouth air. Column conditions were as follows: column temperature 50癈, nitrogen gas flow rate 2 ml/min, hydrogen gas flow rate 14 ml/min, air(l) gas flow rate 17 ml/min, air(2) gas flow rate 10 ml/min. Two members of volatile sulfur compounds family in mouth air, hydrogen sulfide and methyl mercaptan were analyzed qualitatively and quantitatively. After the gas chromatography experiments, pocket deep (PD) and sulcus bleeding index (SBI) of each patient was examined. The results were analyzed with SPSS 10.0 statistical analysis package.[Results] The concentrations of methyl mercaptan, hydrogen sulfide and total sulfur were 3.414 ?3.421 mg/m3, 1.558 ?0.506 mg/m3, 3.743 ?2.684 mg/m3 respectively. The VSCs content in mouth air moderately increased with PD and SBI, the same as the ratios of CH3SH/H2S.Part n : Correlation between Measurements of Volatile Sulfur Compounds in Mouth Air Using Halimeter and GasChromatography[Materials and Methods! Fifty patients of halitosis were randomly selected in halitosis clinic in our hospital. Halimeter was used to determine the concentrations of intraoral headspace volatile sulfurcompounds in the mouth air of each patient. The monitor used in the present study was calibrated beforehand. Each time before measurement, the monitor was zeroed according with the ambient air. Participants were asked to refrain from talking or opening their mouths for 3 minutes before the measurement was taken, and the measurement was performed by inserting a disposable plastic straw approximately 4 cm into the partially open mouth. Results were recorded as peak ppb sulfide equivalents. Every subject was measurement three times, and the means were recorded. Then, a gas chromatography equipped with a pulse flame photometric detector was used to estimate the concentrations of volatile sulfur compounds in mouth air. Evaluating the correlation between measuring results of the two methods was finished with SPSS 10.0 statistical analysis package. [Results] The readings of the halimeter were highly correlated with the values of the total vo...