Experimental Study Of Xylose Improving Gastrointestional Function In Mice

[Objective]The purpose of the study is to research the effects of xylose on gastrointestinal function improving in Kunming mice.[Methods]Experiment 1: Forty Kunming mice were randomly divided into 4 groups, i.e., high, medium and low dose of xylose and the control, 10 cases each group. The mice in the treated groups were orally administered xylose at different dose for 14 days and those in the control were given the same volume of water. The black semisolid nutrient paste was intragastrically given to the mice to see the effect on gastric emptying (GE) and small intestine peristalsis (SIP).Experiment 2:1.Fifty Kunming mice were randomly divided into 5 groups, i.e., the control, the constipation model, and the treated groups at the high, medium and low dose of xylose, 10 cases each group. All groups but control were given diphenoxylate (10mg / kg bw) to established constipation model. Charcoal was given as the indicator. Then the first defecation time, the amount of feces, the weight of wet or dry feces, and water content in the intestine within 6 hours were observed. 2. Forty Kunming mice were randomly divided into 4 groups, i.e., the control, the groups at high, medium and low dose of xylose, 10 cases each group. The effects of xylose on water absorption of small intestine and large intestine were studied by weighing the intestines.Experiment 3: Forty Kunming mice were randomly divided into four groups, i.e., the control, high, medium and low dose of xylose, 10 cases each group. The experimental groups were given xylose and the control were given distilled water for 14 days, andintestinal flora were counted at the end of the experiment.[Results]Experiment 1: The remaining rates of gastric contents of the treated groups were lower than that of the control. Compared with the control, the remaining rate of the gastric contents at the low, medium and high dose group was reduced by 17.88%(P<0.05), 27.17%(P<0.05) and 33.41%(P<0.01), respectively, and the peristalsis function of small intestines was improved. Compared with the control, the rate of small intestinal peristalsis was increased by 21.02%(P<0.05), 25.76%(P<0.01), and 30.76%(P<0.01) in the low, medium and high dose of xylose group respectively.Experiment 2:1.Compared with the model, the first defecation time of low dose of xylose had no statistically significant difference(P>0.05). However, at the doses of 4.2 and 8.4 g xylose / kg bw for 7 days, the first defecation time was significantly shortened (PO.001) ,the amount of feces, the weight of wet feces or dry feces, and the water content in intestines within 6 hours was increased(P<0.01 or PO.001). 2. The water content in small intestine at high dose of xylose group had significant difference compared with the control (P<0.05) . However, there was no statistical difference at low or medium dose of xylose group compared with the control (P>0.05) . All xylose groups could significantly enhanced the water content in large intestine in mice (PO.05).Experiment 3: At the dose of 8.4g/kg bw, the number of Bifidobacterium and Lactobacillus was increased 6.0%(P<0.01), 6.4%(P<0.01) respectively. On the contrary , the number of Enterobacter and Enterococcus, was reduced by 8.1%(P<0.01) and 5.4% (PO.05) , respectively. At the dose of 4.2g/kg bw, the amount of Bifidobacterium and Lactobacillus was increased 4.3% (PO.01) and 4.5% (PO.05), respectively. Meanwhile, the number of Enterobacter was reduced 7.8% (PO.05) , but the number of Enterococcus had no significant change (P>0.05) . At the dose of 2.8g/kg bw, the number of the four kinds of intestinal flora had no significant change (P>0.05 ) compared with the control.[Conclusion]1 Xylose can distinctly promote the ability of gastric emptying and improve the peristalsis function of small intestine in mice.2 Xylose can significantly shorten the first defecation time and increase the amount of feces, the weight of wet feces, and water content. Furthermore, xylose can obviously increase the large intestinal water content in mice, which suggesting that xylose had the action of loosening bowel to relieve constipation.3 Xylose can stimulate the proliferation of Bifidobacterium and Lactobacillus, and inhibit the proliferation of Enterobacter and Enterococcus. These results suggested the xylose could regulate and improve the intestinal flora and could stimulate the proliferation of Bifidobacterium in mice efficiently.4 Xylose can improve mice's gastrointestinal function similar to xylitol and xylooligosaccharides.