Acupuncture Of Different Acupoints Impact Of Differences In Bowel Movements To Inhibit The Electrical Activity Of Rat Small Intestine And Mechanisms Of Research

ObjectiveObserve electricity-acupuncture different points to the influence of small intestine movement by the neuroscience method, from multi-acupuncture points, multi-objective synchronizations system.Compare with the acupuncture big mouse Zusanli, Liangqiu, Neiguan, Daling points under the physiology, the pathological state the small intestine movement influence difference, and discusse its function mechanism from plant nervous system angle.MethodsTake the male Wistar big mouse as the object of study, the atropine intravenous injection makes the mold, then puncture separately Zusanli, Liangqiu, Neiguan, Daling 5min, keeps needle 10min, record big mouse small intestine myo- electricity, the Vagus nerve with the internal organs big nerve electric discharge by the method of electrophysiology; examine NOS(nitrogen monoxide synthase) expression of cross coupling, parasympathetic main center by the method of organization chemical process.Results1. After atropine intravenous injection, the small intestine smooth muscle action potential frequency drops obviously;2. The electricity-acupuncture treatment groups among various acupuncture points subnit group Zusanli Liangqiu, Neiguan, three groups small intestine smooth muscle action potential frequency remarkably mark up around acupuncture (P<0.01) , the small intestine movement's inhibitory state has improve distinctly. Among various acupuncture points subnit group, the group of Zusanli small intestine myo- electricity mark-up scope to be biggest, Liangqiu, Neiguan the next best, and between two points the difference is not obvious. Around the Daling group the small intestine myo- electricity do not remarkably change around acupuncture(P>0.05) .3.The electricity-acupuncture treatment group among various acupuncture points subnit group compares that the advances of the Vagus nerve's discharging frequency has statistics significance around the acupuncture (P<0.05) ; among the comparisons of the multural groups,the Zusanli group to be most obvious, next is the Liangqiu and Neiguan group, the Daling group is weakest. Zusanli, Liangqiu, Neiguan group compares that the reduce of internal organs big nerve electric discharge frequencies has statistics significance around the acupuncture (P <0.05) , the Daling group does not have the remarkable difference around acupuncture treatment. among the comparisons of the multural groups,the Neiguan group of the reduce of internal organs big nerve electric discharge frequencies to be most obvious, next is the Liangqiu and Zusanli group.4.The electricity-acupuncture treatment group the neuron of intestines movementsympathetic nerve main center (the Vagus nerve after dorsal nucleus) has the varying degree dyeing to deepen, especially the colorations of the Zusanli group and the blank group are close. Intestines movement nerve main center (T5-T9 section spinal cord side angle and midgut back, ventralis): In the midgut back, the ventralis and the side angle may see the NOS masculine neuron. In the electricity-acupuncture treatment group, the cytoblastema coloration of Zusanli, Liangqiu, Neiguan subnit group to be shallow, especially the colorations of the Zusanli group and the blank group are close .The difference of the Daling group cytoblastema coloration not to be obvious around the mold.ConclusionThe electricity-acupuncture Zusanli, Liangqiu, Neiguan group can promote the small intestine movement to suppress the model big mouse's intestinal tract smooth muscle electricity activity restoration obviously, causes the activity of the small intestine smooth muscle tends to be normal,the Daling group is not obvious as for intestinal tract's control action;But among the comparison of Zusanli, Liangqiu, Neiguan three points, the Zusanli point is strongest to the small intestine movement function's control action, Neiguan and Liangqiu has the function but weak to the Zusanli point. The Electricity-acupuncture function effect possibly is through the change Autonomic center neuron's function condition, adjustment nervus autonomici activeness, restores confuses -the cross coupling to be balanced, thus realizes to the small intestine movement adjustment.