The Study On In Vitro Differentiation Of Human Mesenchymal Stem Cells In To Neural-like Cells

Over the last few decades, embryonic stem cells and tissue stem cells have been the major objects for study in the field of stem cell engineering . With the development of tissue engineering, cell replacement therapy became a new approach to treat diseases of the central nervous system. The cells from mature tissues can be reprogrammed (transdifferentiated) to change into a completely distinct phenotype from that normally in that tissue. mesenchymal stem cells (MSCs) from human bone marrow(BM) and umbilical cord blood (HUCB) are the typical examples. They can differentiate into adipogenic, osteogenic, chondrogenic, myogenic cells and neural cells.The existence of stem cells with ample proliferation and differentiation potential in umbilical cord blood and bone marrow opens up the possibility of using stem cells found in these tissues to treat degenerative,post-traumatic and hereditary diseases of the central nervous system. This article investigate studies in which cells from postnatal bone marrow or umbilical cord blood were induced to proliferate and differentiate into neural-like cells with Salvia miltiorrhiza. Part ⅠObjective : to investigate the isolation, purification , expansion , cryopreservation and resuscitation of MSCs from bone marrow and umbilical cord blood. Methods: Normal fresh HUCBC and bone marrow were separated with Ficoll-Paquereagent and cultured in Dulbecco's modified Eagle's medium(DMEM) containing 10% fetal bovine serum(FBS), relying on the separation of MSCs by adherence to plastic and passed 4 times, then cryopreserved. The frozen cells were resuscitated after one month. Results: After several passages, the adherent cells became more uniformly spindle-shaped in appearance . The shapes of 2 kinds of cells were same, they were processed to obtain a fibroblast-like population of cells,and they were expanded as undifferentiated cells in culture for more than 10 passages, indicating their proliferative capacity.The frozen cell were resuscitated successfully. Conclusions: MSCs can be obtained from BM and HUCBC.they maintained good puripotantiality and self-renewal capacity and its cryopreservation, resuscitation,could meet with the need of tissue engineering and cell differentiation.Part ⅡObjective : To investigate the differentiation of human mesenchymal stem cells(hMSCs) into neural cells with salvia miltiorrhiza in vitro Results: Salvia miltiorrhiza induced hMSC to exhibit neural morphological characteristics and express neurofilament(NF), βⅢ tubulin and glial fibrillary acid protein(GFAP). Conclusion: The human bone marrow and umbilical cord blood contain stem cells that are capable of differentiate into neural cells, and salvia miltiorrhiza can induce the stem cells to differentiate into neural cells, they may represent an alternative stem cell source for CNS cell transplantation.