Pharmacognostic Study On Herb Of Yi Nationality "DuLa"

"Dula",a herb of Yi nationality often used to prevent from poisoning ,drinking and opium addiction, especially to save people and domestic animals that are poisoned for having eaten the Aconitum ssp. by accident, and its effect is much in evidence.There are twelve plants of the genus Aconitum, family Labiatae and family Dipsacaceae plants in Sichuan and Yunnan. The Aconitum plants are a terrific poison for most of them containing double ester alkaloid. Several Aconitum ssp. Are used as Dula in some zone, if variety is error, it can cause some unnecessary death, so it is very important to learn the original plant of Dula firstly. The more research can be done in the future.Based on the abundant field observation and the collected species,we found that original plant of Dula inclide six families and sixteen species,its main species is Aconitum episcopale Levl. .After researching about characteristic and histology of Aconitum episcopale Levl. ,Aconitum pseudostapfianum W.T.Wang and Geranium platyrenifolium Z. M. Tan, we set up key point for identification of the species.According to traditional way, the extract of Dula was tested for its toxin by method in mice, the extract indicated that the LD50 of Aconitum episcopale Levl. is 54.35g/kg and Aconitum pseudostapfianum W. T. Wang is 7.16g/kg. Six kinds of Dula were as tested for its resist arrhymia by aconitine. The result shows that Aconitum episcopale Levl. , Aconitum pseudostapfianum W. T. Wang and Geranium platyrenifolium Z.M.Tan have certain effect to the arrhythmia.In same method ,we found effect parts were 95% ethanol part and total in five extract parts of Aconitum episcopale Levl.Roots of Aconitum episcopale Levl. was investigated about its chemical component. Cholesterin,Veratric acid, Talatisamine were isolated. There components could be assigned by their IR, MS, ¹HNMR, ¹³NMR. Cholesterin and veratric acid were separated from Aconitum episcopale Levl. firstly.Veratric cid is a kind of effect component that can defend arrhythmia.