The Clinical Significance Of Detection Of IL-10, IL-12 And IL-18 Levels In Patients With Chronic Virus Hepatic Disease

Chronic virus hepatic diseases are related to the turbulence of immunityof the host, usually with the maladjusted level of the cytokines. In this research,IL-10, IL-12 and IL-18 were detected. The results are helpful to estimate thedevelopment and prognosis of the patients. It maybe provide the new thoughtsto the diagnosis of the hepatitis and hepatocirrhosis.We detected the levels of IL-10, IL-12 and IL-18 by double-sandwichELISA in the sera of 66 patients with chronic hepatitis B and hepatocirrhosis.And the control group is comprised of ten health people. Laboratory data wereexpressed as mean±standard deviation and scope. T test was used betweenthe means. Correlation analysis was performed between bilirubin and IL-10,IL-12 and IL-18.Now research shows that the maladjustment between Th1 and Th2 cellsmaybe the pathogenesis of chronic hepatic disease. All kinds of cytokines,such as IL-10, IL-12 and IL-18, participate in the course of chronic hepaticdisease come into being by regulating the activity of Th1 and Th2. If Th1 cellsare in dominance, it'll promote the course of cell immune and eliminate thevirus. But it'll aggravate the damage of the hepatic cells to acute or limitedinfection. If Th2 cells are in dominance, the cell immune will be restrained,and it tends to have durative infection.IL-10 is expressed mainly by Th2 cells. It can induce the incompetence ofT cells and depress the cellular type of immuity. Contemporary, by speedingthe development of the Th2 cells and antibody, IL-10 may lead humoralimmuity. It is thought IL-12 can enhance the Th1 response and depress theTh2 response. It plays an important role in regulating the differentiation of Thcells. By inducing to produce IFN-γ, IL-12 has the function of antivirus. AndIL-18 is an important cytokine discovered these years. It has many biologicalfunctions such as inducing inflammatory damage, immunoloregulation andantivirus. By activating the hepatocyte toxic path induced by TNF-a and FasL,IL-18 plays an important role in the course of liver damage caused byendotoxin. Contemporary, IL-18 is a powerful IFN-inducing factor. IL-18 andIL-12 have the synergism in stimulating the proliferation of T cell, enhancingthe toxicity of NK cells, resisting tumour and inducing immune effector cell toproduce IFN-γ. Various cytokines are not isolated, but harmony. They associate with eachother. IL-12 and IL-18 are the key cytokines during the cellular type ofimmunity and accelerating the cellular immune response. While, IL-10 depressthe cellular type of immunity. Compared with IL-12 and IL-18, IL-10 isopposite in function, but they can regulate the expression of each other. In thisstudy, the samples are grouped according to different clinic types. Byanalyzing the changing-rule of various cytokines of different clinic types, itcan provide the criterion in estimating the development and prognosis of thechronic virus hepatic disease. The results are as following. The levels of IL-10,IL-12 and IL-18 in sera of chronic hepatic disease are obviously higher thanthose of control group (P<0.05). With the levels of TBIL being higher, thelevels of IL-12 and IL-18 go up and the levels of IL-10 go down. There is apositive correlation between the levels of IL-12, IL-18 and TBIL, ALT. InHBeAg positive groups, the activity of IL-10 is higher than those in HBeAgnegative groups. While, the activities of IL-12 and IL-18 are lower. We canconclude these cytokines are related to the degree of the virus replication.Therefore, changes of the levels of IL-10, IL-12 and IL-18 have significance inestimating the development and prognosis of chronic hepatic disease.