The Effect Of Super-high Pressure To Shape,Structure And Function Of HBV

Hepatitis B, which is also called serum hepatitis,viral hepatitis B,is caused by HBV, spread by blood and body fluid, can transform intochronic hepatitis B. Hepatitis B prevails widely in our country,morbidity is very high, in some place, morbidity is above 70%.According as correlative data, there are 189 million people whoseserum HBV DNA is positive in our country, further more there areabout 400 million who shoμld carry through therapy but don't to do so(carrier)in the world. Hepatitis B is the worst infectious disease atpresent, which infect children and young people mainly. Hepatitis Bhas various clinical exhibition, can easily become chronic hepatitisand hepatocirrhosis, some patient can become liver cancer. But, thereis no perfect anti-viral medicine which can cure chronic hepatitis B, so,it is very immportant that invent hepatitis B bacterin.Super-high pressure can kill virus obviously, the function isrelevant with viral broken membrane and depressed absorbility; at thesame time, people also find viral antigenity keep steadiness. Manyscholar utilize super-high pressure biotechnology to produce bacterin,finding new method which can prevent infection. So, although wehave make great progress on studying hepatitis B virus'molecμlarbiology, we have new method that producing hepatitis B bacterin byknowing super-high pressure biotechnology, and that now there is noany report with regard to super-high pressure,s influence on shape,structure and function of Dane's particle and HBV DNA, the studycan work over the affect of super-high pressure to hepatitis B virus. My article mainly study the change of Dane,s particle,globalparticle,tubμlar particle and HBV DNA's shape,structure andfunction under different pressure. We utilize electron microscope toresearch the change of Dane,s particle,global particle,tubμlarparticle,s shape under different super-high pressure, distill HBVDNA ,and then use Southern print hybridization to research if HBVDNA has ruptured, next we use fluid involving Dane's particle,globalparticle,tubμlar particle inocμlates BALB/c mouse, researchingderivational immunoreaction, finding the best controllable conditionmake viral particle after super-high pressure has antigenity but can notcause disease, further more induce effective immunoreaction, in orderto find new method that produce hepatitis B bacterin.at the same time,we contrast using CDV. Method: (1)we subside plasma involvingHBV using (NH)2SO4, and then eccentry plasma to get Dane,sparticle,global particle and tubμlar particle(mixture) (2) dividingmixture into three equal share, dealing with respectively mixtureunder air pressure,350Mpa pressure,450Mpa pressure, (3)researchingDane's particle,global particle and tubμlar particle by electronmicroscope (4) using mixture under different pressure to inocμlateBALB/c mouse, researching immunoreaction (5) distilling HBV DNA,...