| IntroductionOver 80% severe acute pancreatitis patien' s cause of the death is secondary infection of the pancreatic necrosis constitution and apparatus exhaustion correlated with infection . The investigation confirmed that in rats necrosis tissue of acute necrotizing pancreatitis were all cultivated Escherichia coli and other negative Gram staining Bacilli, it is thus evident that controlling the gut origin infection can reduce the death of the SAP. The bottom of the gut origin infection s pathophysiology is intestinal mucous membrane barrier dysfunction. The main cause of intestinal mucous membrane barrier dysfunction is the intestines micro-circulation dysfunction.This study attempted to explore the relations between SAP and intestinal microcirculation, the effect of urokinase on the intestinal microcirculation of SAP by observing pancreas blood flow, the levels of IL - 1(3, and the pathologic changes of intestine on the basis of SAP model in Wistar rats.Materials and Methods1. experimental materialsA total of 192 male Wistar rats ( provided by experimental center of the animal, The Second Affiliated Hospital of China Medical University), weights ranged from 220g to 250g, were randomized into control group(C) pancreatitis group(P) and treatment group(T). each group was divided into subgroup A and B, rats in subgroup A were used to measure intestinal blood flow, and rats in subgroup B, blood samples and intestinal tissue were collected to observe thechanges of IL - 1β levels and intestinal pathologic changes. Each subgroup was divided into four small groups as the phases of 2h, 6h, 12h, 24h after SAP model was finished successfully.2. Experiment methods;2.1 Intestinal tissue blood flow : Radioactive biomicrosphere technique was used to measure the intestinal blood flow at the phase of 2h 6h 12h and 24h.2.2 IL - 1 β levels measurement; To measure the levels of IL - 1 β with the methods of radioimmunoassay ( RIA) , IL - 1β RIA Kit purchased from East -Asia Immunization Technique Institute of the 301 hospital.2.3 Intestinal tissue pathologic histology: Intestinal tissues were embedded in paraffin and sliced into sections, stained by hematoxylin and eosin. Observed the intestinal pathologic changes by pathologist with light microscope, on the principle of blindness.3. Statistics;SPSS11. 5 statistics software was used.Results1. Intestinal blood flow; Comparing with group C, intestinal blood flow was decreased significantly ( P<0.01) in all phases of group P and group T; Comparing with group P , intestines blood flow in2h,6horl2h phase of group T was significant increase(P <0. 01) , but the increase of blood flow in 24h phase of group T was not significant difference( P >0.05 ).2. IL - 1 β level; Comparing with group C, IL - 1 β levels were increased significantly (P<0.01) in all phases of group P and group T, but comparing with group P, IL -1β levels were decreased significantly (P<0.01) in all phases of group T.3. Pathological changes; intestinal tissue has gentle swelling and inflammatory infiltration in group C, and intestinal mucous membrane has great hyperemi-a, inflammatory infiltration, necrosis in group P, and the changes aggravated as the course goes on; Comparing with group C, intestinal tissue has great pathological changes in group T , but the pathological changes improved significantly... |
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