| Bifidobacterium is important inhabitant in intestinal tracts of men and animals. It has beneficial effects on intestinal health. Nowadays development of new source of Bifidobacterium and their application in food industry have been gain worldwide interest. The antioxidant ability of Bifidobacterium was studied by five experiments in vitro and in vivo. They were composed of isolation and identification of Bifidobacterium, study on growth characteristics and growth factor, scavenging effects on free radicals in vitro and the effects on the anti-aging in mice.(1) A large culture collection of Bifidobacterium (61 strains) was screened or activated from infant fecal samples, pig fecal samples, biological preparation and Bifidobactrium conserved in the lab. Using CMRS medium, the tolerance ability of Bifidobactrium was studied in imitating gastroenteric environments. The result shows that gastrointestinal tract tolerance ability of different Bifidobacterium strains was different, the tolerance of A03, A04 and L-1 were best among the tested strains, which have latent ability to use as the strains of Microbial Ecological Agents. At the same time A04 grows better than others, so the strain A04 was chosed and it was identified as Bifidobacterium breve A04.(2) The growth characteristics of B. breve A04, which was tolerant to gastrointestinal tract, were studied. The results showed that it grew best in CMRS medium, their living bacterial number was 9.26 log cfu/cm. Optimal temperature for their growth was 35-37℃, and optimal initial pH was 7.0. The optimal percent of inoculability volume was 3%. B. breve A04 grew fast and it was tolerance to acid. There were not obvious delaying stage in growth curve of B. breve A04, and it entered stability stage after 10 hours.(3) To study the stimulation effect to the growth of B. breve A04, 4 kinds of Oligosaccharide, 8 kinds of Chinese traditional medicine and 4 kinds of food raw materials were used. The results indicate that Soya Bean Oligosaccharide is the most effective (P<0.01) composed to Isomaltooligosaccharide and Fructooligosaccharide, and Stachyose is almost noneffective. When the percent of Oligosaccharide in CMRS is no less than 10%, the growth of B. breve A04 is inhibited. When the percents are 2%, all the Chinese traditional medicines can stimulate the growth. Windflowers, Eleutherococcus Senticosus and Astraglus are the most influential ones, however, different percents showed quite different effects. The food raw materials introduced also activated the growth of B. breve A04, and the better growth compounds are M-5%, CJ-2%, TE-2% and LE-5%.(4) Invitro scavenging effects to inhibiting fatty acid oxidation, DPPH. protecting DNA damage, active oxygen free radicals of B. breve A04, culture supernatant and intracellular cell-free extracts of B. breve A04 is studied. The results show that B. breve A04, culture supernatant and intracellular cell-free extracts of B. breve A04 only can inhibit fatty acid oxidation and scavenge DPPH and active oxygen free radical, but also they can protect against oxidation of DNA from hydroxyl radical mightily.(5) To investigate the effects of B. breve A04, culture supernatant and intracellular cell-free extracts of B. breve A04 on the anti-aging in mice induced by D-galactose. The results showed that B. breve A04, culture supernatant and intracellular cell-free extracts of B. breve A04 could increase significantly the activity of SOD, CAT in serum and could decrease significantly the content of MDA in serum and liver and of lipofuscin and MAO in brain. The results of the experiments suggest that B. breve A04, culture supernatant and intracellular cell-free extracts of B. breve A04 could have anti-aging action with some action mechanisms.
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