| Objective: Diabetic nephropathy(DN) is one of the most hazardous complications on blood capillary of diabetes.It is also the main reason that can cause death and mutilation.Epidemiologic study said: End-stage renal disease caused by diabetes(almost 75% 2 type diadetes) is about 1/3 in American,it is 5% in our country.It has a bad cure rate and high fatality rate . So it effects people's quality of life gravely. Now there is no ideally drug to heal it. The pathology change of DN is insist of kidney hypertrophy , basal lamina thickening, glomeruli extracellular matrix ingredient gathering,et al. For the past few years following the improvement of molecular biology , people discover the factor intra-cellular and etco-cellular taken pleace in cell cycle , which affects the cell hyperplasia and growing.The study about apoptosis(APO) proves that apoptosis participates the DN's mechanisms,and it is one of the important effects that cause renal function more aggravated.Study and research findings, there exists Fas/Fas-L system in the kidney cell, which is participating in control of apoptosis. We use Yushenheji to heal DN and get fine curative effect.We reproduce DN model rats,observe rats'blood sugar, renal function, cell cycle, apoptosis and associated protein Fas/Fas-L, approach the cure mechanism,In order to supply experiment basis. Methods: 10 rats were selected for sham-operation group(SO) randomly in the sixty Sprague-Dawley rats.Others were had right nephrectomy. We injected the rats streptozocin(STZ) intraperitoneally after 2 weaks referinig to 45mg·kg-1.We regarded the rats as model rats whose blood sugar≥16.7mmol·L-1, urine glucose and urine protein were masculine, urinary volume was justo major about 150%.The model rats were randomly divided into:modol group(M), high-dose Yushenheji group(H), Low-dose Yushenheji group(L), Captopril group(C), n=10。H group,L group and C group rats were taken Gliguidone together.All the rats were intragastric administrated 8 weaks.We took rats 24h urine in metabolic cages to mensurat urinary protein(Upro)and urinary creatinine(Ucr).Animals were sacrificed after 8 weaks, to measure fasting plasma glucose(FPG),blood urea nitrogen (BUN),serum creatinine(Scr). The left kidneys were harvested for pathological study.TUNELwas used for observing apoptosis cells . Flow cytometry was used for observing apoptosis cells, cell cycle, Fas/Fas-L. Results: 1 Biochemical effect of Yushenheji on rats 1.1The effect of Yushenheji on the FPG The value of FPG of Group M (20.25±3.14;25.26±4.25)wasraised obviously compared to that of group SO(p<0.01)at every period.At the same time, each treatment group was lower than that of group M(p<0.01).the FPG of group H (12.60±1.75;10.69±2.20) had the therapeutic equivalence compared with that of group C (14.05±2.22;12.16±2.53)(p>0.05).The therapeutic efficacy on group H rats outstripped group L (17.21±2.35;16.31±2.96)(p<0.01). 1.2 The effect of Yushenheji on the 24h Upro content. The 24h Upro in group M increased significantly comparing with group SO at every period (p <0.01),At 6th,8th weeks the treatment groups were lower than group M(9.88±3.14;16.27±3.75)(p<0.01).The 24h Upro content of group L (8.29±1.44; 10.75±4.22) and group C (7.97±1.60;10.09±2.85) were higher than that of group H (6.05±1.34: 6.75 1.33 ) (p <0.05 or p <0.01). 1.3 The effect of Yushenheji on KW/BW The velue of KW/BW of group SO (3.37±0.69) was lower than that of group M obviously(p<0.01).The KW/BW of the treatment groups were lower than that of group M(p<0.05,p<0.01).H group(4.66±0.69) had the therapeutic equivalence compared with group C (4.80±0.85)(p>0.05).The therapeutic efficacy on group H outstripped group L (6.06±0.51()p<0.01). 1.4 The effect of Yushenheji on BUN, Scr and Ccr The velue of BUN, Scr of Group M were raised obviously compared to that of group SO(p<0.01).The treatment groups was lower than that of group M(p<0.01).There had thetherapeutic equivalence compared with every treatment groups(p>0.05). The velue of Ccr of Group SO (3.32±0.48) were raised markedly compared to that of group M(1.14 ±0.37)(p<0.01). Each treatment group was higher than that of group M(p<0.05). There had the therapeutic equivalence compared with every treatment groups(p>0.05). 2 Apoptosis rate, cell cycle, Fas/Fas-L effect of Yushenheji on rats 2.1 Detecting apoptosis rate by TUNEL We found no apoptosis cell in glomeruli of group SO.Each treatment groups apoptosis rate were lower than that of group M(0.94±0.11)(p<0.01).there had the therapeutic equivalence compared with every treatment groups(p>0.05). The apoptosis rate was higher than that of group SO(p<0.01),which was detected in nephric tubule in group M(16.17±0.52).The apoptosis rate of group L(10.30±0.24)was higher than group H(9.94±0.36)(p<0.05),while group C(9.68±0.25) is of no difference with group H(p>0.05). 2.2 Detecting cell cycle by flow cytometry At the stage G0/G1,the number of group M (23.63±11.64)decreased obviously comparing with group SO (66.15±0.50)and the treatment group(sp<0.01). Th evalue of group SO and the treatment groups had no statistical significance(p>0.05). At the stage S the number of group M(44.00±9.66)increased obviously comparing with group SO (14.45±4.03)(p<0.01).Th evalue of group SO and the treatment groups had no statistical significance(p>0.05). At the stage G2M , every groups had no statistical significance(p>0.05). Th evalue of PI of group M is higher than that of group SO (0.24±0.00)and the treatment groups(p<0.01). PI of group SO and the treatment groups had no statistical significance(p>0.05). 2.3 Detecting apoptosis by flow cytometry The apoptosis rate of group SO(3.17±0.43) decreased comparing with group M(p<0.01). The apoptosis rate of group M was higher than that of treatment groups(p<0.01). There had the therapeutic equivalence in every treatment groups(p>0.05). 2.4 Detecting the relative amount of Fas/Fas-L by flow cytometry The relative amount of Fas of group M (1.31±0.03)was higher than group SO(p<0.01). That of the treatment groups decreased comparing with group M(p<0.01).There had no statistical significance in group C (1.14±0.02)and group H(1.15±0.05) (p>0.05),while group L(1.18±0.04)was higher than group H(p<0.05). The value of Fas-L of group M (1.46±0.05)was increased comparing with group SO(p<0.01). The relative amount of Fas-L of the treatment groups deceased comparing...
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