The Effect Of Interleukin-2 On Apoptosis Following Spinal Cord Injury In Rats

Objective: In rats model of spinal cord injury,to explore the interleukin-2 on apoptosis followingspinal cord injury in different time.Methods: 72 healthy rats were chosen and their body weight varied from 250 gram to 310 gram.The rats were divided into two groups randomly:controlled group(36) and interleukin-2 group(36). All of rats spinal cord injury model were established with ALLEN bump equipment(25gcm).The model of IL-2 group injected IL-2(10u) every day until they were sacrificed. The model of controlled group injected NS(0.5ml/d) every day until they were sacrificed. Fabrication of tissue section:after spinal cord injury ld,3d,5d,8d,14d,21d,we sacrificed rats in batch.4% formaldehydum polymerisatum were pured into the cardiac muscle and fixed for 24h.Then obtain the specimen of spinal cord from T₅ to T₁₃.Establishing slices,HE dyeing,bcl-2 dectation,terminal doxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL).On the fourteenth day after spinal cord injury,we detect Gale grading score and tiltboard experiment. Result: Tunel dectation:In control group,after 24 hours,there are many positive cells,most of them are gliao cells,it reached the peak after 3-8 day,and then,they were decreased gradually.in IL-2 group,positive cells are obviously less than those of control group(P<0.05).Bcl-2 dectation:During the first day after spinal cord injury,we find many bcl-2 protein in the spinal cord tissue.In control group,they reached the peak on the third day after spinal cord injury. In IL-2 group,they reached the peak on the eighth day after spinal cord injury.Bcl-2 protein are more in glial cells than those in nerve cells.They begin to decrease after the fourteenth day of spinal cord injury (P<0.05) . The chang of hindlimb motor function on the fourteenth day after spinal cord injury: IL-2 group is not better than control group (P>0.05) .Conclusion: From the investigate,we proved that IL-2 can improve the microcirculation,get rid of oxygen free radical,resist oxygenation,so as to restrain the apoptosis of nerve cell and glial cell,lesson spinal cord injury,improve to recover spinal nerve.Therefore,during early time of spnal cord injury,we make use of IL-2 with other medicine,it can lesson spinal cord injury and provide a new method for treatment of spinal cord injury.