Establishment Of Experimental Models For Hepatic Fibrosis Induced By Thioaetamicle And Ally Alcohol

Objective: To establish experimental models in rats by intraperitoneal injection of Thioaetamicle (TAA) and Ally alcohol respectively. Methods: Of 200 Wistar rats, 40 were injected with 250 mg/kg of TAA intraperitoneally twice a week, another 40 with 46 mg/kg of Ally alcohol intraperitoneally twice a week for 8 weeks and the remaining as control groups. The rats were killed on each of 14, 28, 42 and 84 days later and the following markers were investigated: l)Body weight; 2)Histopathology of the liver; 3)Liver function tests (plasma concentrations of ALT, ALB and T-BIL). Results: l)After 28 days later the body weights of TAA and Ally alcohol groups were significantly reduced in comparison with control groups (P<0.01). And there were no difference between TAA and Ally alcohol groups (P>0.05); 2)At 14 days irregular distribution of hepatocyte, hepatocellular degeneration and necrosis were observed and periportal zone enlarged. That was initiate formation of hepatic fibrosis (P<0.01). Hepatic fibrosis was produced both by 100% in TAA and Ally alcohol groups and none of rats were dead. Hepatic lobular architecture damaged, pseudo lobule was formatted and hepatic cirrhosis was produced. Hepatic cirrhosis was induced both by100% in TAA group and 88% in Ally alcohol group at 42 days and the mortality of the rats was 20%; 3) After 14 days the activities of ALT and T-BIL in plasma were more elevated in both TAA and Ally alcohol groups than that in control groups, the ALB concentration showed gradually decreasing (P<0.01). At 84 days there was significantly difference of elevated T-BIL and degraded ALB concentrations (P<0.01) and however no difference of ALT concentration can be seen between TAA, Ally alcohol and control groups (P>0.05) and there was also no differences of ALT, ALB and T-BIL between TAA and Ally alcohol groups (P>0.05) from the beginning. Conclusions 1) TAA and Ally alcohol may induce ideal practicable models of hepatic fibrosis and cirrhosis in rats. 2) The model established by TAA may be more useful in study of hepatic cirrhosis and portal hypertension. 3) The models induced by TAA and Ally alcohol may be in favor of the observation and study of hepatic fibrosis.