Hepatic Fibrosis Of Rabbit: Model Establishment And Pilot Study Of Imaging Features

Objective: Hepatic fibrosis is an intermediate process of chronic hepatic disease developing to hepatic cirrhosis,even to primary liver carcinoma.Now many investigators found out hepatic fibrosis is also a reversible process,if the patients could be diagnosis and therapy earlier.So earlier diagnosis and correct stage of hepatic fibrosis have important clinical significance.Our experiments would have been searched for new ways of diagnosing hepatic fibrosis with new technology of imaging. Materials and Methods: There were 46 rabbits involved in this study,Carbon tetrachloride was injected intraperitoneally to make the model of rabbit liver fibrosis.The single-level dynamic CT scan and MRI were performed for control group and experimental groups,then we obtained the peak time and peak enhancement of portal vein,liver parenchyma and abdominal aorta in batch and by grade.CT perfusion parameters including hepatic arterial perfusion, portal venous perfusion,total liver perfusion, hepatic arterial perfusion index,distribution volume,mean transit time,and so on,were calculated respectively with deconvolution method of dual in-put one-compartmental model.SE-EPI sequence was used to diffusion weighted imaging.Then we obtained ADC and EADC of liver with software.SE-EPI sequence was used to MR perfusion weighted imaging. We obtained signal intensity of portal vein and liver parenchyma,and calculated relative regional portal blood volume,relative regional portal blood flow.mean transit time,maximum signal reduction ratio and relative hepatic blood volume .After experiment,we killed rabbits,then the changes of hepatic pathology and markers of hepatic biochemistry were observed respectively.