The Effects Of Distal Splenic Vein Hepatic Artery Shunt In Hepatic Cirrhosis Portal Hypertension Animal Model

Objective: To approach the effects of distal splenic vein hepatic artery shunt in hepatic cirrhosis portal hypertension animal model. Providing the rationale and science basis for this technique's application in clinically treatting hepatic cirrhosis portal hypertension patients.Methods: 1,40 health pigs(ba ma,guang xi) were randomly divided into two groups:normal control(n=10) and experimental group(n=30). The experimental group'pigs were randomly divided into two groups:Group A(n=15) and Group B (n=15).2,The experimental animals were used two methods(CCL₄ intraperitoneal injection and oral administration) to construct the hepatic cirrhosis portal hypertension animal model.Taking the correlate datas from this process.3,Establishing distal splenic vein hepatic artery shunt in animal model and observing the effects of stomach spleen region's venous pressure and portal vein's pressure.Injectting the methylene blue through splenic vein to observe staining in liver.Taking correlate experimental data.4,After the operation,we collect the samples to get the message of hepatic function'change in 3 days,1 week and 2 weeks.5.Before we execute the experiment animals in the 4 weeks,we collect samples , measure the splenic vein pressure and main portal vein(MPV) regularly.6.All data processing used SPSS 13.0 statistical package to analyze.Result: 1.Group A:The time of success is 10 weeks and the rate of success is 100 percent.Group B:The time of success is 20 weeks and the rate of success is 80 percent.The experiment certificates that intraperitoneal injection outstrip oral administration.2.The portal hypertension(PHT) animal model'MPV pressure is 29.90±2.73cmH₂O and the splenic vein(SV) pressure is 31.90±2.68cmH₂O.To compare with the normal control ,the discrepancy has statistical significance(P <0.05, n=22).After operation,the MPV pressure is 23.49±1.10cmH₂O and the SV pressure is 35.45±2.88cmH₂O, Compared with prooperation'pressure, the discrepancy has statistical significance(P<0.05).Before the sacrifice of animal, theV pressure is 21.53±1.26cmH₂O and the SV pressure is 23.09±1.36cmH₂O, Compare MPd with prooperation'pressure, the discrepancy has statistical significance(P<0.05).3.After distal splenic vein hepatic artery shunt in hepatic cirrhosis portal hypertension animal model, the spleen venous blood can enter liver through vascular anastomosis way.Methylene blue is injected from the SV,and the liver'coloretur is fine.Other organs and vascular systems are not color.The effect of shunt is good.When the SV pressure is 23.09±1.36cmH₂O,it can keep stable effect of underpressure.The hepatic artery'residue pressure after mutilat the hepatic artery is 13.55±0.84cmH₂O.The dp is 9.55±1.32cmH₂O. 4.Compared with prooperation'patho,the amelioration is not obviously,but the hepatic function is better.Conclusion: 1,The intraperitoneal injection method and oral administration both can success in constructting hepatic cirrhosis portal hypertension animal model,the experiment certificate that intraperitoneal injection outstrip oral administration.2,Distal splenic vein hepatic artery shunt can utility degrade MPV pressure SV pressure.The optimizate SV pressure is 23.09±1.36cmH₂O and the dp is 9.55±1.32cmH₂O.They are primitive motive of keepping underpressure stable.3,The effects of distal end splenic vein hepatic artery shunt in hepatic cirrhosis portal hypertension animal model with Hepatic function,dielectric,blood clotting,blood ammonia and arterial blood gas(ABG) are temple in 1 week after operation,they can recover gradually.The histopathology change of liver is not obviously,but the change of spleen is obviously.4,Our empirical study provid redundant rationale and science according for this shunt in hepatic cirrhosis portal hypertension patients.