Research Of Fas-L,Caspase-3,NF-κB And Hepytocyte Apoptosis In Septic Rat

Objective: To investigate the role of Fas-L, Caspase-3 NF-кB in signal transduction of heptic apoptosis in order to know the mechanism of decrease of liver function. Methods: Wistar rats were subjected to sepsis induced by cecal ligation and puncture (CLP), and the rats were randomly divided into the normal control, septic group. They were sacrificed at 2h, 3h, 4h, 5h after CLP. Serum and liver tissue Fas-L. Caspase-3. NF- кB protein levels and hepatocyte apoptosis were measured at various intervals. Alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) were also determined at the same time. Results: Fas-L. Caspase-3. NF- кB protein levels were significantly elevated compared to the normal control in liver tissue (P<0.01). Meanwhile, serum ALT and LDH were markedly increased compared to the normal control. Under the electron microscope: different morphological disorder of hepatocytes and hepatic sinusoid endothelial cells were observed, mitochondria swelled, apoptosis in CLP 3h group. Conclusion: Liver tissue Fas-L. Caspase-3 protein levels were elevated, which accelerated hepatocyte apoptosis; NF-кB protein levels were elevated as well, which suppressed hepatocyte apoptosis.