The Influence Of Trigeminal Evoked Potential By Cerebral Ischemic Preconditioning Combining With Anisodamine In Ischemia-reperfusion Rat

Objective : Observer the neurprotective effects of cerebral ischemic preconditioning combining with anisodamine in ischemia-reperfusion rat, by recording trigeminal evoked potential(TEP), investigate more effective methods of the cerebro-resuscitation and detective means.Methods: Fourty Wistar rats were randomly divided into five groups (n=8 in each group) made the reversible focal cerebral ischemic-preconditioning model by occluding the middle cerebral artery ,recorded the changes of the TEP after different electric stimulation. Rats in A group(Sham-operated group) only received the operation and recorded theTEP after4 days ; Rats in B group (cerebral ischemia group)received cerebral ischemia for 15 minutes ,then reperfusion for 24 hours and recorded the TEP after occluding the middle cerebral artery for 90 minutes; Rats in C group(anisodamine therphy) occluded the middle cerebral artery for 90 minutes after received anisodamine and recorded the TEP after reperfusion for 24 hours;Rats in D group (cerebral ischemia and anisodamine therphy)occluded the middle cerebral artery for 90 minutes after operation for 3 days, received intraperitoneal injection of anisodamine and recorded the TEP after reperfusion for 24 hours; Rats in E group(ischemia / reperfusion group) occluded the middle cerebral artery for 90 minutes after sham-operating for 3 days.Results: The amplitudes of Nj-P in B> C> D groups, compared with A and E groups , were significant differences (PO.01) ,D group, compared with B and C groups, was no significant difference (P>0.05) .The latency of N| in B. C. D groups, compared with A groups , was no significant difference (P>0.05) , but compared with E groups ,there was significant difference (P < 0.05) . The latency of P in E group, compared with A groups , was significant difference (P< 0.05 ) . The HE staining shew that the damage in B. C. D groups, compared with E groups ,were significantly diminished. Conclusion: The treatment with cerebral ischemic preconditioning combining with anisodamine can notably decrease ischemicalreperfusion injury,and is more effective than the treatment only with cerebral ischemic preconditioning or anisodamine. TSEP is stable . sensitive . convenient,and is effective in detection of cranial nerve.