β Adrenergic Receptor Expression In Human Detrusor Of BOO Patients Derived From BPH

Background and object: Bladder outlet obstruction (BOO) symptoms derived from benign prostatic hyperplasia (BPH) are the most bothersome symptoms in clinic practise. Irritative bladder symptoms are associated with such kind of disease. It is said that β adrenoceptor stimulation is an effective way of relaxing the human detrusor. Methods: We compared the expression level of the β adrenoceptor mRNA including its subtypes in both normal and obstructive bladder smooth mucles of patients. After tissue RNA extraction with Trizol, β AR mRNA was half-quantified using RT-PCR. PCR products were stained with ethidium bromide and quantified with fluorescence image analysis. Results: In control sample, more than 50% of the β AR mRNA was of the β3 subtype, while about 25% was β1 and 25% β2. This change in obstructive samples.All the subtypes almost the same. Compared the samples of the two group, β3 subtype mRNA was decreased rapidly. While the other two subtypes changed only slightly. Conclusion: Our findings indicate a remarkable decrease of β3 AR mRNA expression in the detrusor of obstructive patients, while no signaficent change in β1 and β2 subtype. This may contribute to the changes in their micturition behavior. If these findings are confirmed in more human samples, Targeting this AR may provide a new therapeutic approach to controlling baldder outlet obstruction and also associated with controlling bladder hyperactivity.