Efficacy Of The Action From Three Classes Of Chinese Herbs On Experimental Acute Pancreatitis In Rats

Objectives: To investigate the different single or combined effects of the three classes of Chinese herbs(Qingre Jiedu, Tongli Gongxia, Huoxue Huayu) on severe acute pancreatitis(SAP) in rats. We select the method of orthogonal test. To learn the interaction between or among three classes of Chinese herbs in the management of acute pancreatitis,in order to direct application of Chinese herbs to different clinical acute pancreatitis status.Methods: SAP rat model was made through the injection of Sodium deoxycholate inversely into the common biliopancreatic duct of the rats. And Sprague-Dawley(SD) rats were divided into 9 groups at random. With the help of orthogonal method, kinds of decoctions of single or in combination of Chinese herbs selected from the three classes were made. SAP rats fed with these seven decoctions immediately and 6 hours after this operation were designated as the therapy groups. SAP rats fed with 0.9% sodium chloride were designated as control groupⅠ. Sham operated rats fed with the same quantity of 0.9% sodium chloride were designated as control groupⅡ(sham operate group).Rats were killed after 24 hours of operation. Blood samples were collected for measurement of serum Endotoxin(ET,),Tumor Necrosis Factor α(TNFα,), Amylase (AMY,), Super-oxide Dismutase(SOD,),Xanthine Oxidase(XOD,), Malondialdehyde (MDA,). Pancreatic tissues were taken to study the histopathologic changes, such as the score of edema ,necrosis and hemorrhage.Results: In control groupⅠnecrosis,severe hemorrhage in pancreatic tissue and bloody yellow ascites in the celiac cavity were noticed. Light yellow saponificational spots were found on the epiploon and the common biliopancreatic duct. Edema,congestion and hemorrhage were also found in lungs. Prominent edema and paralysis expanding were also found in stomach and intestines. The liver was swelling and spot bleeding. Prominent swelling and light to dark red in color were found in kidneys. In therapy groups even though there were changes in the above mentioned organs or tissues,the involvement was significantly improved.The level of serum ET,TNFα,AMY, XOD,MDA in rats of control groupⅠwere higher and serum SOD level was lower than those in rats of control groupⅡ(P<0.01). All the indexes in the therapy groups were improved significantly (P<0.05). In analyzing the effects of single herb,Zhi-zi (ZZ) was found the best in improving ET, TNFα,AMY, XOD,MDA and necrosis, Chuan-xiong (CX) was found the best in livability and in the prevention against hemorrhage. Da-huang (DH) was the best in decreasing the severity of edema. In analyzing the effects of double combination, CX+ZZ were the best in decreasing ET, ,TNFα,AMY, XOD,MDA,necrosis,hemorrhage and the livability, increasing SOD. DH+ZZ were the best in decreasing XOD and alleviating hemorrhage. DH+CX were the best in decreasing AMY level. The effects of triple combination were the best in improving all the above mentioned indexes. The ZZ and CX+ZZ combination were better than others in improving the ratio of MDA and SOD,and triple combination was the best. The ZZ and DH+ZZ were better than others in improving the ratio of XOD and SOD,and again triple combination was the best.Conclusions: For single herb,ZZ was better than others. For double combination, CX+ZZ were better than other double combinations. Triple combination was the best of all in treating acute pancreatitis. Therefore,combination of the three classes of Chinese herbs for severe acute pancreatitis might be the best.