| Background and objective: The childhood inflammatory disease can be divided into two types, infectious disease such as pneumonia, and noninfectious disease such as asthma and Henoch-Schonlein purpura (HSP).Asthma is characterized by a chronic airway inflammation in which eosinophils, mast cells and T cells are involved. As a result, the imflammation can cause sensitive people to suffer from an airway hyperresponsiveness and reversible airway constriction. Lots of inflammatory cells participate in the pathogenesis of asthma. HSP is the most common blood vessel inflammation disease in childhood, its main clinical manifestations are nonthrombocyte reducing purpura, arthritis or arthralgia, bellyache, gastroenterostomy haemorrhage and nephritis, and its main pathological characteristics are general small blood vessel inflammation surrounded by neutrophils and eosinophils. The cause and pathogenesis of HSP remain uncertain T cells, neutrophils eosinophils and mast cells all paticipate in the inflammatory process. Because pneumonia is a common disease in childhood, and it is also the first killer in child disease, therefore it is very important to investigate its pathogenesis. Now it is regarded that lots of inflammatory cells, their secretory mediators and cytokines are paticipated in the inflammatory process.In recent years, there are numerous reports about interleukins in adult inflammation, but little is known of cytokine changes in child inflammation. So it is necessary to investigate the correlations between plasma levels of IL-4, -8, -10, -16, -17, -18, IFN-y and eotaxin in child patients with inflammation diseases and their clinical implications.Subjects: Twenty-two child patients with asthma, twenty-one child patients with HSP, twenty-four child patients with pneumonia and 20 healthy controls were recruited into the study. Blood samples from these patients were collected at both acute and convalescent stagesMethods: The plasma levels of IL18, -17, -16, -10, -4, TPN-y and eotaxin were detected by the Sandwich EL1SA procedures.Statistics: All statistical analyses were performed using SPSS (Version 10.0). Data are shown as the mean ?SEM, in all analyses P < 0.05 was taken as significant.Rresult: 1. Plasma levels of IL-18, -17, -16, -10, -4 and IFN-y in child patients with asthma: The data showed that plasma levels of IL-17 were significantly decreased in the patients with asthma at both the acute (P<0.01) and convalescent (P<0.01) stage compared with healthy controls. The plasma levels of IL-16 were significantly decreased in the child patients with asthma at the acute stage compared with the convalescent stage (P<0.05) and healthy controls (P<0.01); but were significantly increased at the convalescent stage in comparison with healthy controls (P<0.01) . In contrast, the plasma levels of IL-10 were significantly elevated in thepatients with asthma at the acute stage compared with the convalescent (P<0.01) stage and healthy controls (P<0.01) The plasma levels of IL-4 were significantly decreased in patients with atopic asthma at the convalescent stage compared with the acute (/><0.05) stage and healthy controls (P<0.05). But the levels of IL-18 and EFN-y had no significant differences between the patient and control groups. At acute stage of child patients with asthma, there were correlations not only between the plasma levels of IL-18, EL-16, IL-4 and IFN-Y, but also IL-17 ^ -1(K -4 and EFN-y.2. Plasma cytokine profile in child patients with Henoch-Schonlein purpura (HSP): the data showed that the plasma levels of IL-10 were significantly elevated and IL-17 were significantly decreased in the patients with HSP at both the acute (P<0.01) and convalescent (P<0.05) stage compared with healthy controls. The plasma levels of IL-8 were significantly elevated in patients with HSP at the acute stage compared with healthy controls (P<0.05), but IL-4, -16, -18, eotaxin and IFN-y had no difference between the patients and controls. There were significant correlations between IL-4, -8, -10...
|
PDF Full Text Request