Expression Of HoxA10 In Acute Leukemia And Its Significance

[Objective] To investigate the expression and significanse of HoxA10 mRNA in acute leukemia patients.[methods] HoxAio level was assessed by reverse transcription polymerase chain reaction (RT-PCR) in 50 patients with acute leukemias , 7 healthy volunteers and 3 patients with ITP (Idiopathic thrombocytopenic purpura) . The regularity of the expression of HoxAio gene in acute leukemia and the relationship between HoxAio level and the prognosis of leukemia was explored. [Result] HoxAio was expressed in all types of acute myelogenous leukemia . HoxAio message is also observed in acute lymphoblastic leukemia patients and part of control groups. 3 normal donors were found not to express HoxAio. The level of HoxAio mRNA of acute myeloid leukemia patients was significantly higher than that of acute lymphoblastic leukemia patients and control group, p<0.01. HoxAio gene appeared to be more strongly expressed in AML-M1 and -M2 subtypes than in AML-M4 and -M5 subtypes , and the gene was notable high expressed in acute promyelocytic leukemia. The number of blast and promyeloid cells in the bone marrow is positive related with the level of HoxA, r=0.635 , P<0.01 . the level of HoxAio of 9 non-responsive patients was higher than thatof 8remission patients , but there was undiffrence between them. P=0.258.[Conclusion] HoxA10 was overexpressed in acute myelogenous leukemia , HoxA10 is a mark in order to differentiate lymphoid leukemia and myelogenous leukemia. It is not a specific gene of cancer than a major transcription factors affecting hematopoiesis. The level of HoxAio was related with load of leukemic cells and curative effect with of patients with acute leukemia. HoxA10 might facilitate the leukemia progression with another cofactors.