Expression And Significance Of PCNA Cyclin D1 And P16 In Transitional Cell Carcinoma Of Bladder

To investigate the expression of PCNA cyclin Dl and p16 intransitional cell carcinoma of bladder and in normal bladder mucous membrane ,and to explore the correlation of these proteins expression and their significance ,and toevaluate the prognosis .Method :PCNA cyclin Dl and p16 expression were studiedin 72 cases of transitional cell carcinoma of bladder and in 6 cases of normal bladder mucous membrane by the immunohistochemical staining method.Results: the positive rates of these three expression products in normal bladdermucous membrane were as follows :0%(PCNA), 0%(cyclin Dl) , 100%(pl6). But in transitional cell carcinomal of bladder the positive rates were 72.58%(PCNA), 64.52(cyclin D1) and 42.0%(pl6). The expression products of PCNA and cyclin Dl were found to be positive correlated to the pathological grade and the clinical staging of BTCC. The expression products of p16 were found to be negatively correlated to the pathological grade and the clinical staging of BTCC. There were significant differences between each two proteins' positive expression [p<0.05(PCNA and cyclin Dl), p<0.05(PCNA and p16),p<0.05(cyclin D1 and p16).Conclusion: There was significant differentiation between the expression ofPCNA, cyclin D1 and p16 in normal bladder mucous membrane and in transitional cell carcinoma of bladder with different pathological grade and clinical staging .The positive rates of PCNA and cyclin Dl in normal bladder mucous membrane were significantly lower than the positive rates in BTCC .The positive rates of p16 in normal bladder mucous membrane were significantly higher than the positive rates in BTCC. The abnormal expression of PCNA , cyclin Dl and p16 have close correlation with biologic behavior of bladder carcinoma.