The Research On The Expression Of Cyclin D₁, Cyclin E And P21～(WAF1/CIP1) In Transitional Cell Carcinorma Of Bladder

Transitional cell carcinorma of bladder (BTCC) is one of the most malignant tumors in China. The primary cause of BTCC is the disorder of cell cycle modulation. In cell cycle the disorder of the transition from G! to S phase plays an important role in tumor development. Cyclin D, Cyclin E and P21WAF1C1P1, which are the mam restrictive factors of the G,^S phase , were studied more frequency in recent years . Cyclin D[ and Cyclin E are the positive controlling factors, and p21wAFi,cipi is the negative controlling factor. Their abnormally expression closely relate to the development of BTCC. The effect and relation of Cyclin Dl5 Cyclin E and p21WAFI/CIPl in BTCC are not clear at present. We used immunohistochemical method to research the expression of Cyclin D1, Cyclin E and P21WAF1/C1P>, then studied then-effect and relation in BTCC. The purpose of this study is to clarify the mechanism of the development of BTCC and to determine whether. Cyclin D1, Cyclin E and p21WAFI/CIPl are important factors in predicting the outcome of BTCC.Materials and Methods1. 50 surgically cystectomied BTCC samples and 10 normal bladder inucosa (NBM) were collected. All of the tissues were fixed in 10% neutral formalin, then embedded in paraffin. Five micron sections were prepared.2. Streptaridin-Peroxidase immunostaning technique were used to examine the expression of Cyclin Db Cyclin E and p2lWAF1/CIP1 in all the tissues.3. Spss 10.0 software wrap were used to analyze the data. The statistical methods were x2 test and exact probabilities in 2x2 tables. There is a statistical significance when P<0.05.Results1. In NBM and BTCC tissues, the positive rates of cyclin D, were 0%(0/10) and 42%(21/50) respectively. There were significant difference between NBM and BTCC (P=0.011 ).In NBM and BTCC tissues, the positive rates of cyclin E were 10%(1/10) and 50%(25/50) respectively. There were significant difference between NBM and BTCC (P=0.033 ).IN NBM and BTCC tissues the positive rates of p21WAF1'CIP1 were 10% (1/10) and 44%(22/50) respectively. There were significant difference between NBM and BTCC (P=0.044 ).2. In pathological grade I, II and III of BTCC, the positive rates of cyclin D, were 80%(12/15), 35%(7/20), 13.3%(2/15) respectively. There were significant difference between grade I and II, so between I and III.In grade I, II, III, the positive rates of cyclin E were 26.7%(4/15),50%(10/20), 73.3%(11/15) respectively. There were significant, difference between grade I and grade III.In grade I, II and III, the positive rates of P21WAF1CIP1 were 73.3%(11/15), 40%(8/20), 20%(3/15) respectively. There were significant difference between grade I and grade III.3. Based on the infiltrating depth, the BTCC were divided into superficial or invasive group. In these two groups, the positive rates of Cyclin D,were 59.1%(13/22) and 28.6%(8/28) respectively, the positive rates of Cyclin E were 27.3%(6/22) and 64.3%(18/28), and the positive rates of p21WAF1 CIP1 were 63.6%(14/22) and 28.6%(8/28). All of them have significant difference.4. In positive expression group and negative expression group of p21WAF1/CIP1, the expressions of cyclin E were 31.8%(7/25) and 64.3%(18/25). There were significant differences between the two groups. There was a negative correlation between the expression of p21wAFi/ciPi and Cyclin E (y=₀ 3223). There were no correlations between the expression of p21WAF1 CIP1 and Cyclin D,, either between Cyclin D, and Cyclin E.Conclusions1. Cyclin D! overexpressed in BTCC. It linked to low histological grade, low stage and probably better prognosis.2. Cyclin E overexpressed in BTCC. It linked to high histological grade, high stage and probably poor prognosis.3. The expression of p21WAF1/CIP1 reduced when BTCC had a high grade or high stage. Loss of P21WAF1/CIP1 linked to high grade, high stage and probably poor prognosis.4. Cyclin D, and Cyclin E may promote the occurrence, development o...