The Analysis Of Apolipoprotein E Polymorphism In Chronic Hepatitis B

Objective1. To explore the relationship among IL-2, IFN-γin sera and HBV load , hepatic function condition and liver fibrosis in patients with chronic hepatitis B (CHB).2. To deserve the frequency distribution of ApoE phenotypes and alleles in chronic HBV infected with different clinical manifestations, and the effects of ApoE gene polymorphism in HBV infective immunity were analyzed.Methods1. The quantity of IL-2 and IFN-γin sera were measured, the RIA and ELISA technique were separately used to detect IL-2 and IFN-γ. 2. The blood genome DNA of chronic hepatitis B were extracted, PCR and TDI-FP technique were separately used to copy the ApoE gene and detection of ApoE gene types. 3. According to the virus load the low-quantity group and high-quantity group was choosed out . According to the hepatic function condition the patients was divided into the chronic CHB Virus taking, light-degree, mid-degree and severe-degree group. According to liver fibrosis condition the cirrhosis group and non-cirrhosis group were divided. According to the ApoE gene polymorphism the patients were divided into the ApoEε2 group, ApoEε3 group and ApoEε4 group.Results1. Compared with the low-quantity group the IL-2 in high-quantity group was significantly decreased (P﹤0.05), there was no difference of IFN-γ between them. Along with the severer of hepatic function the IL-2 quantity was decreased and the IFN-γ was increased. Compared with the non-cirrhosis group the IL-2 in cirrhosis group was significantly decreased and the IFN-γwas increased(P﹤0.01). 2. The allele frequencies of ApoE in the chronic hepatitis B ApoEε3: ApoEε4: ApoEε2 were 0.74: 0.16: 0.10, There was no significant difference in the chronic HBV-infected compared with previously published population data, however, the frequency of the ApoEε4 in light-degree(0.22) was higher than the population data(0.04(0.17),and compared with the CHB mid-degree and severe-degree groups, it was significantly higher (P﹤0.05).The ApoE allele frequencies were similar in cirrhosis group and non-cirrhosis group. Compared with the ApoEε3 and ApoEε2 the IL-2 and TC in ApoEε4 were higher. Conclusions1. The activity of IL-2 shows a beneficial effects on eliminating HBV, the quantity of IL-2 has relationship with liver damage and fibrosis formation. There is no beneficial effects of IFN-γon eliminating HBV, and it can accelerate the liver necrosis and fibrosis formation.2. The high carriage of ApoEε4 allele may lighten the hepatic inflammatory damage through regulate the quantity of IL-2 and plasma lipid levels in sera.