The Clinical Study Of IL-1β,TNF-α,IL-8 In Acute Pulmonary Thromboembolism

Objective: To investigate the changes of serum IL-1β,TNF-a and IL-8 inpatients with acute pulmonary thromboembolism (PTE) and the relationshipbetween these cytokines and the disease.Methods: Radioimmunoassay was applied to measure serum levels of IL-1β,TNF-a and IL-8 in 18 acute pulmonary thromboembolism patients (11 massive, 7minor ) in different time. 20 patients undergoing the fracture of femur bone (oneof risk factors for PTE) and 18 healthy subjects were selected as two controlgroups.Results: (1) on the day of admission, the median concentrations in serum of IL-1β,TNF-a and IL-8 were０．７２２８±０．２６５４　ｎｇ／ｍｌ,１．４０９４±０．４２０７　ｎｇ／ｍｌ,１．３４５±０．３７９５　ｎｇ／ｍｌ,respectively, in the PTE group ; 0.4025±0.1912 ng/ml,1.1415±0.3268 ng/ml,0.9056±0.3308 ng/ml, P<0.05, respectively, in the fracture group　ａｎｄ　０．２２２８±０．１５９４　ｎｇ／ｍｌ,０．９０５６±０．２９２３　ｎｇ／ｍｌ,０．７７２８±０．３３６５　ｎｇ／ｍｌ,　Ｐ＜０．０００１,respectively, in the healthy group. (2) During the next days, IL-1βconcentration showed a gradual decline in patients with PTE from ０．７２２８±０．２６５４　ｎｇ／ｍｌ　ｔｏ　０．２６８８±０．１６５６　ｎｇ／ｍｌ,　 (P <.001), TNF-a concentration declinedfrom １．４０９４ ± ０．４２０７　ｎｇ／ｍｌ to １．０１１３ ± ０．２８９２　ｎｇ／ｍｌ,(Ｐ＜０．００１),ａｎｄinterleukin-8 concentration was relatively constant in time,　　ｆｒｏｍ　１．３４５±０．３７９５　ｎｇ／ｍｌ　ｔｏ　１．１５７５±０．３０７９　ｎｇ／ｍｌ,(Ｐ＞０．０５)．(３) Ｓｅｒｕｍ　ｌｅｖｅｌｓ　ｏｆ　IL-1β,TNF-a and IL-8 ｉｎ　ｐａｔｉｅｎｔｓ　ｗｉｔｈ　ａｃｕｔｅ　ｍａｓｓｉｖｅ PTE　ｗｅｒｅ　ｈｉｇｈｅｒ　ｔｈａｎ　ｔｈｏｓｅ　ｗｉｔｈ　ｍｉｎｏｒ　(0.8609±0.2346 ng/ml vs 0.5057±0.1334 ng/ml, 1.5855±0.4145 ng/ml vs 1.1329±0.2625 ng/ml, and 1.5527±0.3484 ng/ml vs 1.0186±0.4741 ng/ml, respectively, P<0.001).Conclusion: (1) IL-1β,TNF-a and IL-8 may play roles in the pathogenesis ofAcute pulmonary thromboembolism. (2) The association between elevatedIL-1β,TNF-a and IL-8 concentrations and the acute phase of PTE provides IIIevidence for a role of inflammation in thrombosis. (3)Such level of elevation ofIL-1β, TNF-a and IL-8 may be involved in the development of venousthrombosis among patients with leg fracture.