Study On Expression And Correlation Of Vascular Endothelial Growth Factor And Its Receptors In Laryngeal Squamous Cell Carcinoma

Objective:To clarity the role of VEGF and its receptors in laryngeal squamous cell carcinoma development and metastasis by detecting their expression in laryngeal squamous cell carcinoma and the adjacent non-neoplastic laryngeal tissues.Methods:The protein and mRNA expression of VEGF and its receptors(Flt-1 and KDR) were examined by Western-blot and semiquantitative reverse transcriptase polymerae chain reaction analysis in laryngeal squamous cell carcinoma(20 specimens) and the adjacent non-neoplastic laryngeal tissues(18 specimens).Results: The protein and mRNA expression levels of VEGF and its receptors(Flt-1 and KDR) were more abundant in laryngeal squamous cell carcinoma than in the adjacent non-neoplastic laryngeal tissues.The expression of VEGF and its receptors(Flt-1 and KDR) in laryngeal squamous cell carcinoma was significantly higher in lymph node positive group than in lymph node negative group.Positive correlation was observed between VEGF and KDR in the laryngeal squamous cell carcinoma and adjacent non-neoplastic laryngeal tissues.There was no correlation between VEGF and Flt-1 expression.Conclusion:The intense expression of VEGF,Flt-1 and KDR in laryngeal squamous cell carcinoma provides strong evidence linking VEGF, Flt-1 and KDR expression to the angiogenesis associated with laryngeal squamous cell carcinoma.The positive correlation between VEGF expression and KDR expression clearly demonstrates that KDR is the main signaling receptor for VEGF in laryngeal squamous cell carcinoma,the VEGF/KDR system plays a functional role in the progression of laryngeal squamous cell carcinoma.