The Expression Of Integrin α3,β1 Subsuits And E-cadherin Protein In Laryngeal Carcinoma And Its Correlation With Clinical Significance

Objective: The laryngeal carcinoma is very common inotolaryngology. As other carcinomas, the invasion andmetastasis influenced patient's prognosis seriously. For part ofpatients, although don't be found clinical lymphadenmetastasize, but they have had tiny metastasis without beingfound or have dangerous of metastasize in the future. So, it isimportent to study the carcinomal metastatic symbols.Cadherins and integrins just are two major Cell AdhesionMolecules correlating with malignant tumour metastasis. It wasapproved that the lower expression of E-Cadherin promotecancer cell'invasion and metastasis. The α3β1 integrincomplex belongs to β1 integrins. In epithelial tissues, thecomplex'important characteristic is playing a role in adhesionbetween cell and basal membrane. Some scholars found theabnormal expression of α3β1 integrin complex in tumourwas related to its progression and metastasis. In our study, weanalyzed the quantitative expression of E-Cadherin protein andintegrin α3,β1 subsuit by FCM in laryngeal carcinoma tissue.Our aim is to investigate the correlation between the expressionof the three factors and clinical significance in laryngeal 6英 文 摘 要carcinoma, find the carcinomal metastatic symbols which mightpredict laryngeal carcinoma' progression,metastasis andprognosis, and offer theoretic information for choice of clinicaltreatment. Methods 1. Materials 50 laryngeal carcinoma fresh samples wereanalyzed and meanwhile 50 para-carcinoma tissues and 18normal laryngeal mucosa samples were also studied as controls.All subjects are taken from the patients who admitted operationtreatment in the 4th hospital of He Bei Medical University from2002.11 to 2003.11. All samples were fixed by 70% alcohol andstored at 4℃. 2. FCM Method The first labeling antibody aresheep-derived IgG antibody to integrin α3(I-19),rat-derivedIgG2 α monoclonal antibody to integrin β 1(K20) andrat-derived IgG1 monoclonal antibody to E-Cadherin. Thesecond antibody is FLIT-IgG, a sheep-derived polyclonalantibody to mouse. Prepare single-cell suspension,immuneflsorescence label cell. The measure was performed with a flowcytometer(FACC420BD USA). 3. Result judgement Fluorescence Index was defined asthe quantitative expression index of E-Cadherin protein andintegrin α3,β1 subsuit. The FI value more than x ± 2sof FInormal laryngeal mucosa should be consided positiveexpression(E-Cadherin FI>0.88, IFα3 FI>1.10, IFβ1FI>1.12),whereas negative expression. 7英 文 摘 要 4. Statistic methods All data are collected by Excel,analysis was performed using the SPSS 10.0 for windowssoftware, including ANOVA, LSD test, x2 test, Fisher exacttest, group t,t＇test, multiple liner regression analysis, P≤0.05indicating distinct statistic significance, P≤0.01 indicating verydistinct statistic significance. Results 1. The expressin of E-Cadherin protein The quantitative(FI=0.88) expression of E-Cadherinprotein in laryngeal carcinoma tissues is obviously lower than inpara-carcinoma(0.95) and normal laryngeal mucosa(1.00)tissues(P=0.005) (P=0.000), and qualitative(56%) expression oflaryngeal carcinoma tissues is lower than those ofpara-carcinoma (22%) and normal laryngeal mucosa(0) tissues( x x 2 =4.456,P=0.035)( 2=6.269, P=0.012) too. There isn'tdistinct statistic significance between the expression ofpara-carcinoma and normal laryngeal mucosa tissues. In laryngeal carcinoma, the quantitative and qualitativeexpression of E-Cadherin protein in metastasis group(0.82, 0) isobviously lower than in non-metastasis group(0.90, 73.7%)(P=0.000)( x 2=35.380, P=0.000), and in clinical stage Ⅲ,Ⅳgroup(0.85, 30%) is obviously lower than in clinical stageⅠ,Ⅱgroup(0.90, 73.3%) too(P=0.009)(...