| Objective: The gastric carcinoma is the most common malignant tumor in the alimentary tract. Peritoneal metastasis is a frequent type of recurrence after surgery and the reason for the poor prognosis of gastric carcinoma. The tumor cells invasion-metastasis, mainly resulted from the adhesion molecule, and proliferation play an important role in this course. Recently, many researches have reported that cytokine-induced killer cells (CIK), being new adoptive immunotherapy cells, have the characteristics of rapid proliferation, high efficiency and broad spectrum in killing of tumor cells. However, there were few reports about its application on treatment for peritoneal metastasis of gastric carcinoma. CIK cells were cultured in vitro and peritoneal metastasis model for human gastric carcinoma was established by injecting CUM-2MD3 cells into abdominal cavity of nude mice. This research was designed to investigate the CIK cells effect on tumor cell proliferation, apoptosis and expressions of E-Cadherin,β1-integrin in peritoneal metastasis models so as to study the CIK cells mechanism of treating peritoneal metastasis of gastric carcinoma and to provide the theory for CIK cells clinical utilization. Methods: 1 Peripheral blood mononuclear cells of the normal person were separated and cultured in the medium containing CD3McAb , IFN-γ, IL-1αand IL-2 in vitro to produce CIK cells.2 The peritoneal metastasis model for gastric carcinoma was established by injecting OCUM-2MD3 cells into abdominal cavity of nude mice. OCUM-2MD3 cell strain came from human gastric carcinoma.3 At the 21th day after injection, 48 model mice were randomly divided into 6 groups-CIK 1, 3, 5 day group and NS 1, 3, 5 day group. Each group had 8 mice. CIK 1 day group was injected CIK cells into abdominal cavity at first day, CIK 3 day group at first and third day, CIK 5 day group at first, third and fifth day. The density of CIK cells was 1×107/0.2ml. NS groups were injected only 0.2ml physiological saline at corresponding time.4 After 24 hours of the last injection, all mice of each group were killed to observe the change of tumor mass and take tumor tissue from the same position for detection.5 The morphological change was observed by naked eye, light and electronic microscope. With flow cytometry equipment, the rate of tumor cells in G0/G1,S,G2/M stage were respectively detected and PI value was calculated. At the same time, the apoptosis rate and expressions of E-Cadherin,β1-integrin were detected too. The data was organized and processed with SPSS10.0 statistical software. Results: 1 CIK cells could be successfully produced by culturing inthe medium containing CD3McAb , IFN-γ, IL-1αand IL-2 in vitro. 2 The peritoneal metastasis model could be successfully established by injecting OCUM-2MD3 cells in abdominal cavity of nude mice. There were many metastasis tumor nodes in the retina, mesentary, parietal peritoneum and serous membrane. 3 In NS groups, the tumor cells showed active proliferation. But in CIK groups, the necrotic tumor cells were increased and more apoptosis phenomenon was founded by light and electronic microscope.4 Compared with NS groups, the rate of tumor cells in G0/G1 stage of CIK groups was significantly higher than that of NS groups﹙P＜0.05), but there was not greatly changed in S stage (P＞0.05). On the other hand, the rate of tumor cells in G2/M stage and proliferation index were significantly lowed﹙P＜0.05). The CIK cells role was increased following the time﹙P＜0.05). So CIK cells might prevent the tumor cells from going into the cell cycle and restrain their proliferation. 5 The apoptosis rate of tumor cells in CIK groups was significantly higher than that in the NS groups﹙P＜0.01). The role was increased following the time﹙P＜0.01). It could be explained that CIK cells might play an important role in inducing tumor cells apoptosis. 6 Both compared with NS groups and compared in CIK groups, the expression of E-Cadherin of tumor cells... |
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