| Objective: Nowadays cancer has become one of the main diseases that endanger human being's health following with the cardiovascular diseases. According to the statistics of WHO, nearly 700 million people occur the cancer all over the world every year, among which 500 million die from it. In our country, 160 million people and 130 million people die. Therefore to cure the cancer has become one of the focusing problems in the world. The regular therapies are surgical operation, chemotherapy and radiotherapy. The immunotherapy or biotherapy has also become the new therapy, but all the therapies above cause many kinds of side effects. For example, surgical operation is the first method to treat the cancer, but it can not prevent the recurring and the metastasis of tumor; chemotherapy and radiotherapy can give rise to the depression of bone marrow, the digestion obstacle, immunity repression and body weakness, biotherapy only apply for the cancer related to immunity. With the development of traditional Chinese medicine (TCM), the western medicine integrating with TCM is now becoming another method to cure the cancer. TCM can lessen side effects of above therapies through strengthening the function of body immune system, which improve the life quality of the patients and the curative effect. Simple TCM can not eradicate all the tumor cells completely, so TCM as the adjuvant assists the western medicine to cure the cancer. In my research, we adopt the tonifying kidney and spleen recipe(TR) prepared by my supervisor combining with cyclophosphamide to cure the tumor transplanted mice to observe the curative effect, in the same time to explore the molecular mechanism of TR on enhancing the therapeutic effect and lessening side effects in tumor transplanted mice in order to supply the theoretical evidence for the clinical use.Methods: 60 mice, whose weight are 20±1g, are randomly divided into five groups. The normal group (N group): normal feed and give no treatment. The model group (M group): with 0.5ml sterile saline being injected into abdominal cavity, and 0.4ml distilled water being poured into stomach. TR group: with 0.5ml sterile saline being injected into abdominal cavity, with 0.4ml the recipe being poured into stomach. CY group: with 30mg/kg of 2%CY being injected into abdominal cavity, with 0.4ml distilled water being poured into stomach. TR+CY group: with 30mg/kg of 2%CY being injected into abdominal cavity and 0.4ml the recipe being pours into stomach. The S180 tumor cells, which transferred of culture about seven days in abdominal cavity, been taken out and washed two times by D-Hank's. The number of the tumor cells is adjusted to 6.25×106/ml. The tumor cells are transplanted in the right armpit of each mouse of the last four groups by 0.2ml. On the second day, the medicines begin to be given once a day for ten days. The common state was observed every day. On the second day of the last time, the weight was weighed, the number of leukocyte was counted with caudal vein blood. The tumor was taken out and weighed. The spleen was taken out and the MTT was used to measure the proliferation activity of lymphocytes. Apoptosis of the tumor tissue was measured by DNA ladder analysis, and the relative expression of P53 mRNA and BCL2 mRNA was examined by RT-PCR. Another 40 mice, whose weight are 20±1g, are randomly divided into four groups. The normal group (N group): normal feed and give no treatment. TR group: with 0.5ml sterile saline being injected into abdominal cavity, with 0.2ml the recipe being poured into stomach. CY group: with 30mg/kg of 2%CY being injected into abdominal cavity, with 0.2ml distilled water being poured into stomach. TR + CY group: with 30mg/kg of 2%CY being injected into abdominal cavity and 0.2ml the recipe being pours into stomach. Three days later, the blood serum was collected after two hours of treatment. The S180 tumor cells, which transferred of culture about six to eight generations, and the number was adjusted to 5.0×106/ml, add into 96 pore plate. The s...
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