The Association Between Coronary Heart Disease And Genotype Of Interleukin-1 Family

Objective: To study the immune modulation, interleukin-1 family genotype and the change of serum interleukin-1 level, in coronary heart disease caused by inflammatory. To offer the laboratory data for discovering the pathogenesis of coronary heart disease. To explore the highly individuational mechanism about clinical semiotics of coronary heart disease, then to search for more pertinent preventive, diagnostic and therapeutic methods.Methods: The interleukin-1 family genotypes were detected in 236 healthy Chinese Han people and 245 coronary heart disease patients by polymerase chain reaction and restriction fragment length polymorphisms methods, synchronously , the plasma levels of IL-lα IL-β IL-IRa and lipoprotein were measured in all control and coronary heart disease patients by enzyme-linked immunosorbent assay and turbidimetermeasure.Results: There are five gene mutation locations, IL-lα C-889T> IL-lα C-511T IL-lα C+3953T\ IL-IRa T+8006CU IL-IRa VNTR, which is consistent with foreign reports. The distribution of IL-1 family genotypes in Chinese Han population is similar to Japanese, but is significantly different from American and German. The serum IL-lα level in patients with acute myocardial infarction is much higher than that in patients with stable angina, unstable angina and control. The serum IL-lRa/IL-lβ ratio in patients with unstable angina and acute myocardial infarction is much lower than that in controls(P<0.01 or P<0.05). The genotypes of IL-la C-889T, IL-IRa T+8006C and IL-IRa VNTR are tightly correlative to coronary heart disease, and the distribution of IL-la C-889T genotype is evidently different between myocardial infarction and angina. The coronary heart disease patients carrying CT or TT genotypes were at increased risk with an odds ratio of 3.19 for myocardial infarction. There is a complete linkage disequilibrium between IL-IRa (VNTR) and IL-IRa (+8006T/C), and the allele II of IL-IRa (VNTR) is always found with allele C of IL-lRa(+8006T/C). The serum IL-la level in patients with CT genotype of IL-lα(-889) isremarkably high than that with CC genotype, and the serum IL-IRa level in patients carrying allele II or C of IL-IRa is markedly high than that with other genotypes. There is no correlation between IL-1 family genotypes and serum TC, TG, HDL-C, LDL-C, ApoAI and ApoB level in Chinese Han population.Conclusions: The distribution of IL-1 family genotypes is evidently race and region different in Chinese Han population. The change of serum IL-lcu IL-lRa/IL-lp may behave with the immune state, which might be available indexes for the state monitoring of coronary heart disease. The IL-1 a C-889T genotype may be the new risk factor of coronary heart disease in Chinese Han population. The gene mutation of IL-1 a C-889T IL-lRaT+8006C and IL-IRa VNTR may influence the serum IL-1 a and IL-IRa levels. However, the IL-1 family genotypes are not the main factor to influence the lipid metabolism.