| Objectives1. To investigate the relationship of Haptoglobin gene polymorphism on pathogenesisand pathologic processes of premature coronary heart disease and analyze its relationshipwith the relevant inflammatory markers, look for the risk factors and the action mechanismof premature coronary heart disease in order to provide a theoretical basis for its preventionand control.2. To explore the plasma levels change of haptoglobin, high sensitivity c-reactionprotein and interleukin-17on premature coronary heart disease patients and healthycontrols, discuss its relationship with the severity of coronary artery stenosis in order toprovide relatively objective indicators for its monitoring.Study populations1. CHD group:92coronary heart disease patients (male age44～55years, femaleage51～62years, mean aged52.4±3.549years) were consecutively collected from thehospitalized in the department of cardiology, the Qingdao municipal hospital from March2010to December2011. All patients were confirmed by coronary angiography. Allpatients were undergoing the Judkins law selective coronary angiography multi-positionprojection. CHD patients were confirmed by coronary angiography based on clinicalmanifestations, at least one coronary artery was blocked more than50%. The Gensini scoreassesses the severity of CHD and produce there different score teams: A group score<50, Bgroup score≥50<90, and C group score≥90.2. Control group:72control group subjects (aged from43to60years, mean aged51.27±4.638years) were selected from the physical examination center of Qingdaomunicipal hospital in the same time. All subjects were confirmed through the medicalhistory, physical examination, laboratory tests, X-ray and ECG to exclude coronary arterydisease, hypertension, various cardio-cerebral vascular disease, diabetes and livers and kidney dysfunction, and required no family history of CHD.Methods1. Hp genotype testing: Peripheral venous blood samples were collected in themorning after an overnight fast for2ml, EDTA anticoagulant, placed in a centrifugecentrifuged at1800rmp for10minutes, isolated from the plasma. Subjects blood DNA wasextracted from the peripheral blood leukocytes by blood genomic DNA extraction kitproviding by TIANGEN Biotech Co. Reference by Werner Koch, the PCR-SSPtechnology is used to explore the Hp genotype.2. Plasma levels of Hp, hs-CRP, IL-17: The concentration of serum Hp and IL-17were detected by ELISA, reagents were purchased from Shanghai Bogoo BiotechnologyCo (monoclonal antibody source of U.S Abcam). The levels of serum hs-CRP weredetected by nephelometry by Germany Dade Behring Marburg GmbH automatic proteinanalyzer BNPROSPEC type.Results1. The Hp2-2genotype frequency between CHD and control group’s respectively is43.48%、54.05%, difference non-statistics significance(P=0.376).The Hp2-2genotypefrequency of A、B、C group is28%、50%、77.78%,C group of Hp2-2gene frequency ishigher than A group obviously（P<0.001）. And the Gensini score of Hp1-1、Hp1-2andHp2-2genotype in CHD group respectively is22.50±13.844、47.05±25.504、65.52±40.580，Hp2-2genotype score is higher than Hp1-1（P<0.001）.2. Plasma level of Hp, hs-CRP and IL-17have a significant differences between thePCHD group and the control group, the PCHD group’s is higher than the control’s(P<0.05). But the plasma concentration of Hp, hs-CRP and IL-17have no significantdifferences among the different sub-group of PCHD group according the Gensini score(P>0.05). The plasma concentration of Hp and hs-CRP, IL-17and hs-CRP show significantcorrelation, the correlation coefficients were0.774and0.665(P<0.001).Conclusion1. The Hp2-2genotype may be not the independence risk factor for the prematurecoronary heart disease, but can relate with the coronary stenosis degree.2. There is a high expression of Hp, hs-CRP and IL-17in premature coronary heartdisease patients, combined detection may be significant to the diagnosis, assessment,treatment and prognosis of PCHD, but lack of predictive value to coronary artery stenosisdegree. |
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