The Study Of Helicobacter Pylori And Precancerous Lesion Of Stomach And Gastric Cancer In Animal

Objective To investigate the relationship of Helicobacter pylori(Hp) and precancerous lesion of stomach and gastric cancer in animal .Methods Sixty of two-grade C57BL/6 little female rats were randomly divided into Hp group and Hp+MNNG group and MNNG group and comparison group . In 12 weeks after the rats of Hp group and Hp+MNNG group were infected with SS1 Hp strain , the Animals of Hp group and MMNG group begin to receive 100μg/ml of MNNG in the drinking water for five months . The other groups keep on drinking distilled water for five months. All animals were sacrificed after the above treatment . Histopathological feature of the glandular stomach were assessed by HE staining . Results By the end of the study , all rats were survival . Hp was detected in all infected rats .The precancerous lesion had developed in two experimental rat in the Hp+MNNG group . Adenomatous hyper plasia were observed in two groups , but its incidence in the Hp+MNNG of experiment were 40℅ , higher than that of the MNNG group (6.67℅) (P<0.05) .The incidence of atrophic gastritis in Hp group and Hp+MNNG group were 66.67℅ ,80℅ respectively , significantly higher than that of the MNNG group and comparison 20℅ , 0 respectively (P<0.01) , however in gastric body no difference (P>.05) . Conclusion We demonstrated that long-term Hp infection could induce the chronic active and atrophic gastritis in animal model , increase the sensitivity of glandular gastric mucosa to carcinogen , and promote the incidence of pre-carcinomatous lesion .