The Association Between A 894Gâ†'T Polymorphisims At Exon 7 Of Endothelial Nitric Oxide Synthase Gene And Diabetic Nephropathy With Or Without Hypentension

Objective: To investigate the association between a 894G - T polymorphisims at exon 7 of eNOS gene and diabetic nephropathy with or without hypertension. Methods: A case-control study for 147 Chinese subject (including 52 type 2 diabetes mellitus, 45 diabetic nephropathy and 50 diabetic nephropathy with hypertension) was performed. The allele frequency and genotype distribution of a 894G-T polymorphisims at exon 7 of eNOS gene were determined by polymerase chain reaction combind with restriction fragment length polymorphism (PCR-RFLP) methods .Results: The frequency of T allele and TG genetype at exon 7 were significantly higer in DN group than that in DM group; but there was not significantly difference between DN with hypertension and DN without hypertension. Multivariate logistic regression analysis showed that the systolic blood pressure, TG, TC, HbAlc and the 894G-T polymorphisims at exon 7 of eNOS gene were risk factors for diabetic nephropathy. Conclusion:T allele of the 894G-T polymorphisims at exon 7 of eNOS gene is related to diabetic nephropathy in type 2 diabetes mellitus, and it is not related to hypertension in diabetic nephropathy.