The Research Of Treatment Of HTS In Traumatic Brain Injury

Objective: To investigate the effect of early administration of HTS post traumatic brain injury on secondary lesion formation and provide possible theoretical basis for HTS-based therapy.Methods: The injury was induced using Feeney's 'freefall' model of traumatic brain injury. The experimental animals were then divided into 6 groups and immediately received intravenously varied dosages of either HTS or normal saline. All animals were sacrificed 6 hours post injury and were injected with EB (i.v.) 2 hours prior to their termination. To examine the potential effects of HTS on brain edema, secondary lesion formation and the integrity of blood brain barrier, we measured the water content of the injured and uninjured control hemispheres, as well as the leukocyte infiltration and EB content in the injured areas. In addition, plasma sodium concentration at various time points post HTS administration was also determined. Results:1.Determination of water in the cerebrum: compared with 0.9% saline group and 7.5%Nacl 2ml/kg group, there were significant decreasing water in both injured and uninjured sides of cerebrum in 7.5% Nacl 4ml/kg group, which was with significant difference (P<0.05); compared with normal group and false experiment group, there were increasing water in injured side and decreasing water in uninjured side(P<0.05),respectively; however, there was difference but inadequate to reach statistical significance between 4ml/kg and 6ml/kg group.2.Counting of leukocyte in injured areas: there was marked decrease of leukocyte in 7.5% Nacl 4ml/kg group in contrast to 0.9% saline group and 7.5%Nacl 2ml/kg group(p<0.05), but with 6ml/kg group, there was of no significant difference (P>0.05). However, compared with normal group and false experiment group, the number of leukocyte in 7.5% Nacl 4ml/kg group was apparently increased (P<0.05).3.Determination of EB content in injured areas: the content of 7.5% Nacl 4ml/kg group was lower than that of 0.9% saline group, 7.5%Nacl 2ml/kg and 6ml/kg group(P<0.05), but higher than that of the normal group and false experiment group(P<0.05).4.Determination of plasma sodium: plasma sodium of every time phase in HTS group was higher than other groups(p<0.05),in which 6ml/kg group was higher than other two groups, with significant difference(p<0.05), and 4ml/kg group was higher than 2ml/kg group(p<0.05). There was decline of plasma sodium with time phase in HTS group.Conclusion: HTS works by reducing water content in uninjured areas, prohibiting aggregation of leukocyte in injured areas and protecting BBB. The administration of HTS by 4ml/kg is the optimal choice.