The Effect Of Low Dose Glibenclamide On Secondary Lesion Of Rat With Acute Spinal Cord Injury

Objective: 1.To investigate the mechanism of the secondary lesion after spinal cord injury.2.To study the protection of Glibenclamide on the secondary lesion after spinal cord injury.Method: To set up the rat experimental model with acute spinal cord injury.84 SD rats were randomly divided into control group, spinal cord injury group, treatment group with GLIB.To observe the pathological change of injured spinal cord by using HE staining ; to observe the cell ultra- structure change on spinal cord after injured using electron microscope, The expression of sur1 in the rat spinal cord was detected by immunohistochemistry staining at 45min, 6h, 24h, 3d and 7d ; to quantitative analyze the expression level of sur1 with ipp6.0 software ; to evaluate the regain to the function of hind limb on rats by using BBB score.Result: HE staining : bleeding of tissue ,proliferation of gitter cell and the neutrophil infiltration in treatment group were more gently than the injury group . observation by electron microscope: invasion of inflammatory cells, lamellar structure of myelin damage, mitochondrial swelling in treatment group are more obviously eased than injury group. Immunohistochemistry staining :the rats expression of sur1 for 45 minutes didn't express and the expression of sur1 in other treatment groups were more feeble than the same time point in injury groups .The expression of sur1 in injury groups would reach to the peak 24 hours after operation .The expression of sur1 in the treatment group was obviously weaker than that in the injury groups . The differences among the three groups at different time point were statistical significance.BBB score showed that the distinction between the injury groups and the treatment groups at 24 hour haven't statistical significance .The distinction among three groups for one weeks have statistical significance. BBB score of treatment group obviously surpassed to the injury group ,but inferior to the control group. Blood sugar tests: blood sugar treatment group declined slightly. In each group ,there are no statistical significance. Conclusion: 1.The sur1 in capillary endothelium controlling the patency of the NCca-ATP channels would be related with the secondary lesion of spinal cord injury .2. There is a protective effect that closing NCca-ATP channels by blocking SUR1 recepto with Glibenclamide.