Expression And Clinical Significance Of P73 And P53, P21～(waf1), PCNA Protein In The Different Diseases Of Gastric Mucosa

Objective To research expression of P73 and P53, P21waf1 ,PCNA protein in the different diseases of gastric mucosa . To investigate the relation between expression of P73 , P53, P2rwaf1, PCNA protein and the malignant transformation of gastric mucosa epithelium and their correlation in gastric carcinoma and the relation to histological grade , lymph node metastases, depth of invasion of gastric mucosa , TNM stage in gastric carcinoma. Methods Immunohistochemical method (S-P method) was used to detect the expression of P73, P53, P21waf1, PCNA protein in 39 cases of gastric carcinoma, 17 cases of chronic atrophic gastritis and 40 cases of chronic inflammatory polyp and a statistical analysis was made according to clinicopathogical data Results The positive rate of expression of P73 protein in gastric carcinoma was markedly higher than that in chronic inflammatory polyp and that in chronic atrophicgastritis (p<0. 05) . The positive rate of expression of P73, P53, P21wat1 protein in gastric carcinoma were, respectively, 64. 1%, 64. 1% and 20.5%. Compared with that in chronic inflammatory polyp group and chronic atrophic gastritis group , the difference were significant (P<0. 05) . As for expression of P21 waf1 protein, the difference was significant between the chronic inflammatory polyp group and chronic atrophic gastritis (P<0. 05). There was a positive correlation between the expression of P73 and PCNA protein (P<0. 05). The positive rate of expression of PCNA protein was 79. 5% in gastric carcinoma Compared with that in chronic inflammatory polyp group, the difference was significant (P<0. 05) . There was no correlation between expression of P73, P53 , PCNA protein and histological grade, depth of invasion of gastric mucosa , TNM stage, lymph node metastases (P>0. 05) . there was negative correlation between expression of P21 *"" protein and TNM stage, lymph node metastases (P<0. 05) , no correlation of expression of P21waf1 to histological grade and depth of invasion of gastric mucosa (P>0. 05). Conclusion Probably high expression of P73 gene is one hot, spot gene alteration of gastric carcinoma and expression of P73 proteinn gastric carcinoma may play an important role in the course of cancerous development. As one tumor suppressor gene, P73 has its tissular specificity. Cooperative expression of P73 and PCNA protein could be an important index to judge the malignant transformation and clinicopathological process and prognosis. P21 waf1 may be an early signal of gastric carcinoma or one of indexes to judge prognosis.