Influence Of Psoriatic T Lymphocytes On Epidermal C-myc, Bcl-2 And P53 Protein Expression In Vitro

Objective: To reveal psoriatic T lymphocytes action on epidermal c-myc, bcl-2 and p53 protein expression in order to investigate the mechanism that psoriatic T lymphocytes induce epidermis to proliferate abnormally. Methods: Jmmunohistochemistry method was performed to detect the expression of c-myc, bcl-2 and p53 in psoriatic lesions, uninvolved skin and normal skin from healthy controls. Normal skin and psoriatic uninvolved skin were incubated with psoriatic T cells for 3 day and 6 day stained for c-myc, bcl-2 and p53 expression, in comparision with expression before culture and in cultured psoriatic uninvolved skin, cultured normal skin and normal skin cultured with normal T cells at the same time. Results: There were significent overexperssion of c-myc and p53 protein in psoriatic lesions, but bcl-2 expression was rarely abserved. The expression of psoriatic uninvolved skin was as nomal. P53 expression in normal skin and psoriatic uninvolved skin was stronger after the 3rd day cultured with psoriatic T cells compared with that before culture and in other controls at the same time. There were significent up-regulation of c-myc expression after the 6th day compared with that before culture and the 3rd day after culture and other controls at the same time, the expression of bcl-2 was decreased, and p53 expression was similar to that cultured after the 3rd day. There was no difference between normal skin and psoriatic uninvolved skin stimulated by psoriatic T cells respectively. Conclusions: C-myc, bcl-2 and p53 protein expression are abnormal in psoriatic lesions, while those in psoriatic uninvolved skin are nomal. The results show that the abnormal expression of the proteins are very important in psoriatic epidermal hyperplasia and abnormal pattern of cellular differentiation. Psoriatic T lymphocytes can influence c-myc, bcl-2 and p53 protein expression in normal skin and psoriatic uninvolved skin, which might be central to initiation of psoriasis. Psoriasis is a immunological disease in which pathogenic T cells rather than epidermal keratinocytes are of primary importance.