Impact Of The Iron Chelator On Mitochondrial Oxidative Stress Of Ventilator-induced Lung Injury Of Oxidative Stress

Object:We studied the damage of mechanical ventilation injury pathologically in rate lung caused by iron chelator deferoxamine(DFO)and the reactive oxygen species(ROS)change in the mitochondrial of alveolar macrophages(AM)and alveolar type ? epithelial cells(AT ?).It aim to have a further discussion of new treatment and a possible mechanism of the mechanical ventilation lung injury(VILI).Methods:The healthy male clean 24 standard(SD)rats,whose weight 300±25g,were divided into 3 groups(n=8),nomarl group(NC group),hyperventilation group of large VT(HV group),Deferoxamine treatment group(DFO group).DFO group,with 200mg/ml dfo intraperitneal injection.HV group,with 0.9%NS intraperitneal injection.Respectively,the 18 animals were killed in each group,to ovserve the level of Reactive Oxygen Species in bronchia alveolus lavage fluid and type II pneumonocyte.Lung tissue specimens were to compute wet/dry wight ratio(W/D)for evaluating the pulmonary edema degree.Observed tissues pathological changes by light microscopy.Results:1.ROS level:Both MFI of macrophage and type II pneumonocyte decrease in NC group and DFO group,compared with HV group,there was difference(P<0.05).2.Wet and dry weight ratio:HV group had an decreasing of W/D value,compared with NC group,there were statistically difference(P<0.05).compared with HV group,the W/D of DFO group had a lower W/D value(P<0.05);3.The pathomorphologic changes:by the light microscope,NC group had a nomal lung pathology images,The alveolar epithelium partially desquamated and fibrinous exudate was found in HV group,compared to DFOgroup,the evaluation of pathomorphologic changes had difference(P<0.05).Conclusion:In the rat mode of VILI,the intervene of iron chelator DFO could reduce the lung damage in pathological efficiently and reduce the ROS emission both in the mitochondrial of alveolar macrophages and alveolar type II epithelial cells.It gave us the idea that iron metabolism may take part in the production of ROS in mitochondrial in VILI which had a role in lung protection in some extend,and the mechanism needs further research.