| Objective: To investigate whether melatonin (MEL) have the recovering effect for experimental block of rat's opticnerve. Methods: To select 30 Wistar rats whose weight are about 200g. Their age and gender are unlimited. They are divided into two groups, experimental group (â… ) and control group (â…¡), after being made into animal model of high intraocular pressure by affusing pressurizing in anterior chamber. Every group is divided into 3 teams according to experimental days, 1 week (â… A and â…¡A), 2 weeks (â… B and â…¡B) and 4 weeks (â… c and â…¡c). Group I is injected melatonin alcohol solution into abdominal cavity immediately after being made into animal model of high intraocular pressure. Then gave the same dosage every two days. Control group was given equal normal saline solution. To inject 30% horse radish peroxidase (HRP) into bilateral corpusgeniculatum, 24 hours before the end of observation period. Eyeballs were extirpated after 24 hours. Then retina was peel off,oxidized by H2O2 and was colorized by tetra methyl benzidine. The number of dyed retinal ganglion cells (RGC) was accounted with optical microscope. And infrastructure of the opticnerve was observed by electron microscope. Results: The number of dyed RGC in group I is larger than that in group II. The electron microscope observation indicated that the opticnerve ultrastructure of the experimental group is better than that of the control group in the same term day. Conclusion: Melatonin (MEL) has the recovering effect for experimental block of rat's opticnerve. |
