| Objective : The glaucoma is very high causing the blind eyes disease of a kind of incidence of disease, it results in depending on the pathology foundation that the function damages is the losing of going on death and optic nerve fibre of retina neuromere cell (RGCs). The death of RGCs often leads to the fact that depends on the function is damaged , the death way to already generally acknowledge RGCs at present is to wither to die. Subject this draw support from normal position end mark law (TUNEL),through observe ginkgo leaf injectio wither on the influence that dies to rabbit chronic high intraocular pressure model retina neuromere cell, affirm the ginkgo leaf injectio flees and has certain regulation and control function to the withering of RGCs, and then prove the ginkgo leaf injectio depends on to the glaucoma that there is protective action in nervous lesion, it is possible to offer for the fact that screen the traditional Chinese medicine clinically to the research that the glaucoma optic nerve protects.Method : Choose 40 adult rabbits of health, weight is 2. 0-2. 5Kg, the male and female are regardless of. Whether make mould choose rabbit right eyes. Make rabbit, mould of success divide into thiophene feel at ease group, ginkgo leaf group, use medicine group and model contrast group jointly at random, 10 every groups, begin to treat in 72 hours after making the mould. Thiophene feel at ease group spend 0. 5% thiophene feel at ease eyes liquid some eyes, once a day, 2 drops each time. Ginkgo leaf group is along rabbit ear reason intravenous injection ginkgo leaf injectioO. 5ml/Kg, once a day. Above-mentioned two kinds of methods will be shared to jointly use medicine in the group, once a day. Model contrast group will not any treat. Measure every group' s intraocular pressure after treating for 5 days, then every group fetches 2 and tests rabbits at random, every group fetch 2 each every 5 days afterwards, ear reason vein pour into air embolism put to death, go to straight skin suture make a reservation, horn sclera reason office cut go cornea, One tests eyes and is cut open and divided into two along the level below the optic nerve after exhausting the iris, crystal,vitreous body,one make all mirror sample is it change to observe retina every pathology, one make electric mirror sample observe cell's ultra micro-structure,another test eyes make TUNEL measure with normal verse cell counting. Result:1. Change of the intraocular pressure: Model contrast group and ginkgo leaf group experiment rabbit eyes in high intraocular pressure state all the time(P>0.05), feel at ease group, use medicine group compares jointly with thiophene(P<0. 01). Thiophene feel at ease group and use medicine group after one one day not treating, the intraocular pressure has already been dropped to the normal range jointly, and there is not difference in two groups of intraocular pressure value (P>0. 05).2. The normal festival cell counts: Thiophene feel at ease group and model contrast group for significance difference(P<0. 01). Ginkgo leaf injectio wither and having certain regulation and control function to die to chronic high retina neuromere cell that intraocular pressure cause, it and model relatively have significance difference(P<0. 01),Do not relatively have a significance difference to feel at ease in the group with the thiophene(P>0.05). Thiophene is it have certain protective action to optic nerve to feel at ease, exist a large number of verse cell wither and die still, the group relatively has a significance difference(P<0. 01). Therefore identification, ginkgo leaf injectio is it depend on to chronic high intraocular pressure the nervous lesion protects therapeutical function to lead to the fact.3. The pathology of every storey of retina changes: Model contrast group rabbit eyes retina all organization obviously become thin, the structure is disorderly, level unclear, section cell figure reduce in a large amount,kernel layer, outside nuclear layers of cell interval obviously expand, the cell arranges disorderlily;After tre... |
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